We narrowed to 11,805 results for: NSI

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pGEX-4T-1-3xFLAG-HSP27

Plasmid

#129229

Purpose

Encodes FLAG tagged human HSP27 for purification by GST affinity

Depositor

Kevin Janes

Article

Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

Insert

HSP27 (HSPB1 Human)

Tags

FLAG and GST

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

Plasmid

#140086

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro-T2A-BFP

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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MARIO/ pcDNA3

Plasmid

#124838

Purpose

mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

No tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGGAselect

Plasmid

#195714

Purpose

pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1-SNAP

Plasmid

#223576

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shTBK1_3185

Plasmid

#167294

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA TBK1 (TBK1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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HA-TAZ-S89A

Plasmid

#32840

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Tags

HA

Expression

Mammalian

Mutation

S89A

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-CKAR2

Plasmid

#236098

Purpose

Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyn-ExRai-CKAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pKD237-3a-2

Plasmid

#90957

Purpose

Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23100, Bba_J23105, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pArcCreER

Plasmid

#46389

Depositor

Liqun Luo

Article

Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

Inserts

Arc (Arc Mouse)
CreERT2

Use

Mouse Targeting

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Expression

Yeast

Promoter

tetO7 promoter

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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GRK2-K220R

Plasmid

#35403

Depositor

Robert Lefkowitz

Article

Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Mutation

K220R

Promoter

CMV

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ACE2-GFP

Plasmid

#154962

Purpose

pcDNA3.1-ACE2-GFP

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

ACE2-GFP (ACE2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-Mito-TurboID

Plasmid

#200963

Purpose

3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Mito-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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