We narrowed to 22,833 results for: Sis

1. pLoxP-DHFR-mCherry

   Plasmid

   #70147

   Purpose

   Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

   Depositor

   David Sibley

   Article

   Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

   Insert

   a selectable dihydrofolate reductase-thymidylate synthase marker
   

   Use

   Expression in toxoplasma gondii

   Tags

   mCherry

   Promoter

   DHFR 5' UTR

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. BICDN-CIBN

   Plasmid

   #102253

   Purpose

   Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   BICDN-CIB1 (CIB1 Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK-HA-TRAF5

   Plasmid

   #58315

   Purpose

   HA-tagged mouse TRAF5

   Depositor

   Ying Zhang

   Article

   Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

   Insert

   TNF receptor associated factor 5 (Traf5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. ULK1-Halo HRD

   Plasmid

   #207548

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15-His-∆NGlpG

   Plasmid

   #155131

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert codes for GlpG residues 87-276

   Promoter

   T7

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB435

   Plasmid

   #74825

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CAG-REV1-p2A-GFP-PuroR

   Plasmid

   #153001

   Purpose

   Overexpression of human REV1

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   REV1 (REV1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP1354

   Plasmid

   #119275

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. B2094_CRY2olig-miRFP

   Plasmid

   #215114

   Purpose

   Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CRY2olig-miRFP (CRY2 Mustard Weed)
   

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8e-dimer

    Plasmid

    #138490

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Flag TSC2 (T1462A)

    Plasmid

    #14130

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 T1462A (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T1462A

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pEN716 - pCAGGS-eGFP-3Xnls-bpA

    Plasmid

    #156435

    Purpose

    For transient expression of nuclear eGFP in mammalian cells

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    eGFP
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1neo-14-3-3-CLuc

    Plasmid

    #107611

    Purpose

    measure LATS activity by Luciferase Assay

    Depositor

    Xiaolong Yang

    Article

    Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

    Insert

    pCDNA3.1hygro-BamHI-CLuc1433-NotI
    

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB543

    Plasmid

    #74877

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-TRAIL-R1

    Plasmid

    #61382

    Purpose

    Expresses HA tagged TRAIL receptor 1

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 1
    

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R1 leader peptide (aa1-23) replaced with TN…

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3_2FLAG-AKAP79ΔPKA

    Plasmid

    #183452

    Purpose

    Expression of human AKAP79 lacking the PKA binding site (Δ391-400)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa391-400 deleted

    Promoter

    CMV

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1 Utr230-EGFP

    Plasmid

    #58472

    Purpose

    Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Utr230 (UTRN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_FLAG-Mma PylRS

    Plasmid

    #154762

    Purpose

    plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-GFP (F64L, S65T, V163A)-TRP1

    Plasmid

    #53184

    Purpose

    chromosomal tagging with stable GFP

    Depositor

    John Pringle

    Article

    Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP (F64L, S65T, V163A)

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

20. 10XSTAT92E–luciferase

    Plasmid

    #37393

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    SOCS36E enhancer fragment (Socs36E Fly)
    

    Use

    Luciferase; Jak/stat reporter construct

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHLuc Control

    Plasmid

    #141091

    Purpose

    pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CFP-PBD

    Plasmid

    #11394

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    p21-activated kinase 1 (PAK1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

23. R26-H2B-mCherry HR donor vector

    Plasmid

    #137928

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-mCherry-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDEST mfap4:icre-p2a-tomato

    Plasmid

    #135217

    Purpose

    Cre coexpressed with tdTomato in macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter with iCre and Tomato
    

    Use

    Cre/Lox; Zebrafish expression

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. UAS-u(M)Cas9

    Plasmid

    #127384

    Purpose

    Expresses Cas9 at medium level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(M)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pWPI-E2F3

    Plasmid

    #114298

    Purpose

    Constitutive expression of the E2F3 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    E2F3 (E2F3 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV-FLEx-TetOn3G-3xHA-FLEx

    Plasmid

    #133729

    Purpose

    Plasmid contains the floxed TetOn3G-transactivator sequence for expression of TetOn3G in the presence of Cre recombinase.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    TetOn3G-3xHA (floxed & inverted)
    

    Use

    AAV

    Tags

    3xHA-tag

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-N2-RIPK3

    Plasmid

    #78822

    Purpose

    To detect localization of RIPK3 protein in live mammalian cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Tags

    yomRuby3

    Expression

    Yeast

    Promoter

    none

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lyn-mCherry-GAI(1-92)

    Plasmid

    #37315

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    Lyn and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

32. MSCV eIF4E IRES GFP

    Plasmid

    #18761

    Depositor

    Scott Lowe

    Article

    Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

    Insert

    eIF4E (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

33. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-6P-1-HsRCK_E

    Plasmid

    #146210

    Purpose

    Bacterial Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    HsRck (DDX6 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGWB412

    Plasmid

    #74806

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Dual opposing T7 promoters reporter plasmid

    Plasmid

    #156457

    Purpose

    A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Insert

    NeoR/KanR
    

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEG302 22aa SunTag VP64 gRNA4 (FWA)

    Plasmid

    #120249

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHis-hMTMR1

    Plasmid

    #100723

    Purpose

    Expression of human MTMR1 PH-PTP domain

    Depositor

    Byung Il Lee

    Article

    Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

    Insert

    MTMR1 (MTMR1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 95-607

    Promoter

    T7

    Available Since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 3410 pBM272 Bcl-XL

    Plasmid

    #8772

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

    Insert

    Bcl-XL (Bcl2l1 Mouse)
    

    Expression

    Yeast

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge