We narrowed to 59,446 results for: cloning

1. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mouse NHERF-1

   Plasmid

   #32950

   Depositor

   Edward Weinman

   Article

   Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

   Insert

   Slc9a3R1 (Nherf1 Mouse)
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   t3

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBE45

   Plasmid

   #174655

   Purpose

   CAPTURE method receiver plasmid for Streptomyces heterologous expression

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBE44

   Plasmid

   #174654

   Purpose

   CAPTURE method 15A origin of replication receiver plasmid

   Depositor

   Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL50009

   Plasmid

   #50309

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   C terminal HA tag (6x Human influenza hemagglutinin)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-MARK4_IDG-K

   Plasmid

   #189884

   Purpose

   Gateway destination clone of MARK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MARK4 (MARK4 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB301-2μ-HDV

   Plasmid

   #196329

   Purpose

   A yeast-Escherichia coli-Agrobacterium shuttle vector

   Depositor

   Zhenghe Li

   Article

   Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB700 lenti gRNA Blasto

   Plasmid

   #167904

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICSL30008

   Plasmid

   #50300

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   N terminal HA tag (6x Human influenza hemagglutinin)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. DUX4 pDonor221

    Plasmid

    #187801

    Purpose

    DUX4 entry clone

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p667-UBC-CDC42BPA-V5-miniTurbo_IDG-K

    Plasmid

    #189907

    Purpose

    Gateway destination clone of CDC42BPA (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDC42BPA (CDC42BPA Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p663-UBC-miniTurbo-V5-CDK20_IDG-K

    Plasmid

    #189883

    Purpose

    Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK20 (CDK20 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-BrainbowTEC

    Plasmid

    #82405

    Purpose

    Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Inserts

    tdTomatoMYC fusion
    EGFP
    E2CrimsonHA
    

    Tags

    HA and MYC

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6_N7-sgRNA2

    Plasmid

    #190664

    Purpose

    Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    N7 sgRNA2
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMEXC3H

    Plasmid

    #110080

    Purpose

    Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFLAG_OriM

    Plasmid

    #110097

    Purpose

    Analogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL50004

    Plasmid

    #50316

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mCherry variant of RFP (Discosoma sp.)
    

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. p663-UBC-miniTurbo-V5-DYRK4_IDG-K

    Plasmid

    #158184

    Purpose

    Gateway destination clone of DYRK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DYRK4 (DYRK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICSL30003

    Plasmid

    #50304

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
    

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBSDONR P1-P4

    Plasmid

    #71744

    Purpose

    ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Use

    Gateway entry vector for use in multisite gateway…

    Available Since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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