We narrowed to 9,085 results for: mel

1. pTol2p2A--3mnx1:mTagBFP-NS-MCS

   Plasmid

   #78250

   Purpose

   Can be used to subclone in gene of interest into blue motor neuron expression vector.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Inserts

   -3mnx1 (mnx1 Zebrafish)
   -mTagBFP-NS-MCS
   

   Use

   Zebrafish motor neuron expression

   Promoter

   -3mnx1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

   Plasmid

   #178883

   Purpose

   Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   U6-Nla repeat-BbsI-Nla repeat
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLKO-mcherry-luc-puro-Renilla cDNA

   Plasmid

   #29783

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   Renilla
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

   Plasmid

   #175509

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CHMP6_GFP

   Plasmid

   #31806

   Depositor

   Daniel Gerlich

   Article

   Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

   Insert

   CHMP6 (CHMP6 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-CMV-hAAT-W

   Plasmid

   #126704

   Purpose

   Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Insert

   hAAT (SERPINA1 Human)
   

   Use

   Lentiviral

   Promoter

   CMVp

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERroGFP_iE_C204Q_pCDNA3

   Plasmid

   #48629

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B, C204Q

   Available Since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBP-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199201

   Purpose

   Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   split-intein-Gal4[1-20]-N-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN3P-HA-Suv4-20h2

    Plasmid

    #86691

    Purpose

    For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
    

    Tags

    HA

    Promoter

    T3

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DIO-TVA-V5-RG

    Plasmid

    #119743

    Purpose

    Expresses TVA, V5, and RG in a cre dependent manner.

    Depositor

    Marie Carlén

    Article

    Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

    Insert

    TVA, V5, Rabies Glycoprotein
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGK:HygroR-CMV:mKate2-B_UTR

    Plasmid

    #68480

    Purpose

    Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Hygromycin Resistance
    mKate2-B_UTR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    shRNA sensor cassette "B" added to 3&#x…

    Promoter

    CMV and pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-NBL10

    Plasmid

    #69971

    Purpose

    Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

    Depositor

    Jeffrey Friedman

    Article

    Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

    Insert

    NBL10
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pRedCm

    Plasmid

    #176225

    Purpose

    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PrhaB and PL

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-SpyCas9

    Plasmid

    #113034

    Purpose

    AAV vector for expression of SpyCas9 in mammalian cells

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SpyCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid

    #175507

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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