We narrowed to 9,027 results for: mel

1. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

   Plasmid

   #172066

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pUBC_V5_MS2-mVenus

   Plasmid

   #107606

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag

   Depositor

   John Rinn

   Article

   Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

   Insert

   MS2-mVenus
   

   Use

   Synthetic Biology

   Tags

   V5 and mVenus

   Expression

   Mutation

   MS2: V75E/A81G

   Promoter

   human UBC

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRB17

   Plasmid

   #52527

   Purpose

   serves as template for PCR-mediated fusion of U6-promoter with sgRNA

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Drosophila U6C promoter
   

   Use

   CRISPR; Blunt cloning kit

   Tags

   T7-promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE4-SsAPOBEC3B R54Q

   Plasmid

   #138344

   Purpose

   Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-SsAPOBEC3B R54Q
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. roGFP_iE_C147S_pQE30

   Plasmid

   #48631

   Purpose

   Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 147 to Serine mutation (roGFP C147S, E147b, C204)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   roGFP1
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   insertion E147B, C147S

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM0638

   Plasmid

   #129005

   Purpose

   Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCOLA-Gent-yibDp-Erv1p

   Plasmid

   #202483

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-BE4-PpAPOBEC1 H122A

   Plasmid

   #138338

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1H122A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 H122A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Change of His122 to Ala.

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGK:HygroR-CMV:mTagBFP2-A_UTR

   Plasmid

   #68479

   Purpose

   Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mTagBFP2-A_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "A" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Nov. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pSws1-nfsB-CFP

    Plasmid

    #86375

    Purpose

    for zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase (nfsB bacteria)
    

    Use

    Zebrafish

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175506

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. F-CDK8L

    Plasmid

    #24762

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

    Insert

    F-CDK8L (CDK19 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUBC_HA_PP7-mCherry

    Plasmid

    #107605

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mCherry
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    hUBC

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-mGreenLantern/EGFP

    Plasmid

    #179913

    Purpose

    All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-GAS41

    Plasmid

    #15428

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    GAS41 (Yeats4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only