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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 12,758 results for: sequence

Showing: 5261 - 5280 of 12758 results

  4. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    91565-plasmid-map-sequence-id-456463

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  6. HisMS2_VLP_RSV

    Plasmid
    #175958
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Inserts
    P gene
    M gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175958-plasmid-map-sequence-id-357255

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  10. AICSDP-82:SON-mEGFP

    Plasmid
    #133964
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON
    Depositor
    Allen Institute for Cell Science
    Insert
    SON Homology Arms with mEGFP-linker (SON Human)
    Use
    CRISPR
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133964-plasmid-map-sequence-id-264342

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  11. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Use
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  13. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  14. pBabe puro IkB alpha M

    Plasmid
    #12332
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  15. N1-Mito-pHCountdown

    Plasmid
    #182303
    Purpose
    mammalian cell plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence
    Depositor
    Andrew York
    Article
    Seidel et al (2021), doi: 10.5281/zenodo.5810930
    Insert
    mitochondria targeted pH countdown
    Use
    Tags
    COX8A mitochondria targeting sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    182303-plasmid-map-sequence-id-360540

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  17. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Use
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  18. pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

    Plasmid
    #148113
    Purpose
    Mammalian Expression of HshnRNPA2-B1
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPA2-B1 (HNRNPA2B1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    splicing variant of hnRNPA2 variant B1. Exon 6 an…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148113-plasmid-map-sequence-id-396914

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Showing: 5261 - 5280 of 12758 results