We narrowed to 17,206 results for: puro

1. pSIQ-EGFP

   Plasmid

   #216239

   Purpose

   All-in One IQ transgene switch for EGFP expression

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-eCas9-sgMIB1

   Plasmid

   #140239

   Purpose

   Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   sgRNA targeting human MIB1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLENTI_RPS3

   Plasmid

   #127128

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS3 (RPS3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FL tension sensor module

   Plasmid

   #101170

   Purpose

   Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

   Depositor

   Carsten Grashoff

   Article

   Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

   Insert

   YPet(short)-FL-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi_RP_MicroID2-FLAG-NES

   Plasmid

   #188525

   Purpose

   Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   MicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-TIS11B CPM2

   Plasmid

   #121143

   Purpose

   Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLEG(R1-R3) #BGV149

   Plasmid

   #48956

   Purpose

   Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pMRXIP-GFP-STX17ΔNTD

   Plasmid

   #89941

   Purpose

   Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

   Insert

   human syntaxin17 lacking N-terminal domain (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   deleted amino acids 1-161

   Promoter

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSin-GFP-Fg

   Plasmid

   #174307

   Purpose

   Lentiviral expression of GFP

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   GFP (eGFP Aequorea victoria)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiCRISPRv2-KLF7-1

    Plasmid

    #169797

    Purpose

    Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for KLF7 neighbouring region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDA027

    Plasmid

    #131385

    Purpose

    ORFeus expression, mutant

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    ORFeus
    

    Use

    Tags

    ORF2-3xFlag

    Expression

    Mammalian

    Mutation

    H230A, D702Y

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT PUR mGSK3AWT

    Plasmid

    #35159

    Depositor

    Jim Woodgett

    Article

    Doble et al Dev Cell. 2007 Jun;12(6):957-71.

    Insert

    GSK-3α (Gsk3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. LentiCRISPR-sgPREX1

    Plasmid

    #86127

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    gRNA targeting PREX1 (PREX1 )
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-SI-121

    Plasmid

    #131126

    Purpose

    A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPM-D4 miR30-MORC2 ts1

    Plasmid

    #115886

    Purpose

    MORC2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    MORC2 shRNA (MORC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sg14x(MS2) MUC4.1

    Plasmid

    #101153

    Purpose

    gRNA with 14 MCP binding site

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MUC4 (MUC4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

    Plasmid

    #85714

    Purpose

    Telomere-targeting Nme sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CRISPR V2 sgMTHFD1L_1

    Plasmid

    #106316

    Purpose

    Express Cas9 and sgRNA targeting MTHFD1L

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD1L
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTurquoise2 WC visual barcode

    Plasmid

    #158669

    Purpose

    tagging clonal populations with whole cell mTurquoise2 fluorescent protein

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNoTag-DGCR8

    Plasmid

    #164694

    Purpose

    Express DGCR8 without tag in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lentiCRISPRv2-TTF-1-gRNA

    Plasmid

    #101857

    Purpose

    Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    gRNA targeting TTF-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MCP-mCherry-TIAL1

    Plasmid

    #207669

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIAL1

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    TIAL1 (TIAL1 Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ZapLck biosensor

    Plasmid

    #131584

    Purpose

    Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

    Insert

    ZapLck biosensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRIPZ-Myr-PTEN

    Plasmid

    #206983

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    N-terminal myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EYFP NES visual barcode

    Plasmid

    #158676

    Purpose

    tagging clonal populations with NES EYFP fluorescent protein. Adding NES to EYFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEG(R1-R4) #BGV057

    Plasmid

    #48957

    Purpose

    Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pQCXIP TBRII

    Plasmid

    #19147

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    Transforming growth factor, beta receptor II (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pMRXIP GFP-Vti1b

    Plasmid

    #45922

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pLKO human shRNA 1 DEPTOR

    Plasmid

    #21335

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 13. ():.

    Insert

    DEPTOR shRNA 1 (DEPTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pMXs-IP-EGFP-ULK1(K46N)

    Plasmid

    #38197

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Asparagine

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

31. mTurquoise2 Peroxisome visual barcode

    Plasmid

    #158671

    Purpose

    tagging clonal populations with Peroxisomal mTurquoise2 fluorescent protein.

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. shLacZ

    Plasmid

    #42559

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shLacZ
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

33. IRAK1-UTR

    Plasmid

    #15095

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    IRAK1 3'UTR (IRAK1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

34. mTAGBFP2 NES visual barcode

    Plasmid

    #158667

    Purpose

    tagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTAGBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CLPIT Cry2PHR-mCh-RhoA

    Plasmid

    #42959

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Off promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. p663-UBC-miniTurbo-V5-CDK13_IDG-K

    Plasmid

    #135266

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRetroSuper-shSrc

    Plasmid

    #26982

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Src (SRC Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

38. TRAF6-UTR

    Plasmid

    #15093

    Depositor

    David Baltimore

    Article

    Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

    Insert

    TRAF6 3'UTR (TRAF6 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pAPM-D4 miR30-TRIM28 ts3

    Plasmid

    #115864

    Purpose

    TRIM28 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    TRIM28 shRNA (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid

    #189889

    Purpose

    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge