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Plasmid#228508PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I5CHO (#1)
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZB578_pB1H1_T7-RNAP_LARP-I
Plasmid#228507PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3428 MTK234-LacO-BtoApegRNA
Plasmid#226669PurposeMTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNADepositorInserthU6-LacOS-BtoApegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-LacOSAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3331 MTK234-TetO-AtoBpegRNA
Plasmid#226668PurposeMTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNADepositorInserthU6-TetO-AtoBpegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-TetOAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C63S
Plasmid#225080PurposeExpresses precursor peptide ChrA-C63S in E.coliDepositorInsertChrA-C63S
UseTagsHis6ExpressionBacterialMutationC63SPromoterT7Available sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM
Plasmid#207650PurposeOverexpression of native L-cysteine desulfidase in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-decR
Plasmid#207653PurposeOverexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655DepositorInsertdecR
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPP4
Plasmid#209966PurposeContains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmidsDepositorInsertPromoter (P40)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-EV
Plasmid#209973PurposeContains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmidsDepositorInsertCDS (mock)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
UseTagsExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3474
Plasmid#215370PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3473
Plasmid#215246PurposeCDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathwayDepositorInsertLuz
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3472
Plasmid#215245PurposeCDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathwayDepositorInsertH3H
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro
Plasmid#211616PurposeRhno1 Plk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmycExpressionMammalianMutationS51A, T52A, S141A, S162A, S163A, S164A, S178APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC Destination
Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD043
Plasmid#212913PurposeEncodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSCW4 (SCW4 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD084
Plasmid#212921PurposeEncodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertTIP1 (TIP1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD001
Plasmid#212898PurposeEncodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD008
Plasmid#212905PurposeEncodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertPLB1 (PLB1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only