We narrowed to 15,748 results for: pET

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MAC_N_FEV

Plasmid

#167757

Purpose

Expresses FEV in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

FEV (FEV Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#186452

Purpose

used as source for acquisition of new spacers

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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p3R112C/T180CBM3

Plasmid

#85090

Purpose

Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The R112C/T180C variant of PCNA3 fused to P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

Promoter

T7 promoter

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMW96

Plasmid

#178066

Purpose

Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

Depositor

Tarun Kapoor

Article

Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

Insert

CDK5RAP2 (CDK5RAP2 Human)

Expression

Bacterial

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-ALIX(P801G)

Plasmid

#180025

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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RGD-sfGFP-I27-CC

Plasmid

#85392

Purpose

MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling

Depositor

Khalid Salaita

Article

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-868, P801G)-Strep

Plasmid

#164444

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

Insert

GB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Mutation

Proline 801 to Glycine

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZF-AAA

Plasmid

#127465

Purpose

coding sequence for wild type zinc finger ZF-AAA (ZiF268)

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AAA (EGR1 Human)

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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DENV_1ED3

Plasmid

#251246

Purpose

To express the dengue virus serotype 1 envelope protein domain III in E. coli

Depositor

Yutaka Kuroda

Article

Islam et al Int J Mol Sci. 2023 Jan 25;24(3):2393. d

Insert

DENV_1ED3

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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H1N1-PR8_RBD

Plasmid

#251261

Purpose

To express the H1N1-PR8 influenza receptor binding domain in E. coli

Depositor

Yutaka Kuroda

Article

Tu et al Int J Mol Sci. 2024 Apr 1;25(7):3943. do

Insert

H1N1-PR8_RBD

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_2ED3

Plasmid

#251247

Purpose

To express the dengue virus serotype 2 envelope protein domain III in E. coli

Depositor

Yutaka Kuroda

Article

Islam et al Int J Mol Sci. 2023 Jan 25;24(3):2393. d

Insert

DENV_2ED3

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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MZB127

Plasmid

#217836

Purpose

Bacterial expression of human Arf1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf1, rtxA (ARF1 Human, Vibrio cholerae)

Tags

10x His and CPD

Expression

Bacterial

Mutation

Q71L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MZB160

Plasmid

#217841

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf1 (ARF1 Human)

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Mutation

L8K, Q71L

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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H75R SUMO1

Plasmid

#221060

Purpose

Expression of H75R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H75R

Promoter

T7

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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E67K SUMO1

Plasmid

#221065

Purpose

Expression of E67K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35K SUMO1

Plasmid

#221055

Purpose

Expression of H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35K site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35K

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35S SUMO1

Plasmid

#221056

Purpose

Expression of H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35S site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

H35S

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67R SUMO1

Plasmid

#221064

Purpose

Expression of E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67R site mutation on SUMO 1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

E67R

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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