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pGA1-EF1α-sfGFP150TAG-4xtRNA

Plasmid

#251517

Purpose

Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021

Insert

sfGFP150TAG

Tags

His6 and V5

Expression

Mammalian

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

EF1alpha

Available Since

March 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_blast_FAM136A_sg2

Plasmid

#244876

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_puro_FAM136A_sg1

Plasmid

#244877

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgOMA1 sg2

Plasmid

#244866

Purpose

Knockout of human OMA1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

OMA1 sgRNA (OMA1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_blast_FAM136A_sg1

Plasmid

#244875

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_puro_FAM136A_sg2

Plasmid

#244878

Purpose

Knockout of human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A sgRNA (FAM136A Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgOMA1 sg1

Plasmid

#244865

Purpose

Knockout of human OMA1

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

OMA1 sgRNA (OMA1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-mGrm3-WPRE

Plasmid

#224236

Purpose

AAV expression of mGrm3 from GfaABC1D promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mGrm3 (Grm3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Flag- Src H492A

Plasmid

#246425

Purpose

Expresses Src mutant H492A (aka H495A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Histidine 492 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src H201A

Plasmid

#246424

Purpose

Expresses Src mutant H201A (aka H204A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed His 204 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src E524A

Plasmid

#246426

Purpose

Expresses Src mutant E524A (aka E527A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Glu 527 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src C185A

Plasmid

#246423

Purpose

Expresses Src mutant C185A or C188A in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Cys 188 to Ala

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 K26/27Q IRES Cherry

Plasmid

#228112

Purpose

Lentiviral expression of human INTS4 K26/27Q

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Lysine 26 and 27 to Glutamine

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5R 15xGly-uFMR1-3xHA

Plasmid

#242191

Purpose

Transient expression of 15xGly-uFMR1-3xHA

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

15xGly-uFMR1-3xHA (FMR1 Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLJY1 DD-FAM98B[1-330]

Plasmid

#211353

Purpose

Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B[1-330] (FAM98B Human)

Use

Lentiviral

Tags

FKBP12 DD and V5

Mutation

Truncation of C-terminal glycine-rich IDR

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR1 Cys-less

Plasmid

#236274

Purpose

Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

Depositor

Vsevolod Gurevich

Article

Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

Insert

arrestin-1 (SAG Bovine)

Expression

Bacterial

Mutation

C63A, C128S, C143A, inserted GGT codon after star…

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73

Plasmid

#231726

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12deltaCTD(1-1082)

Plasmid

#231735

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(1-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK12

Plasmid

#231716

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK13

Plasmid

#231717

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK13 (CDK13 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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