We narrowed to 13,758 results for: Lor;

1. pJS-BCD-Strep-SAVED-CHAT-cbm

   Plasmid

   #214032

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   K121E, Q122A, N274A, R276E, Y285A, F286A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS-BCD-Strep-dPCaspase

   Plasmid

   #214035

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H79A, C146A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS-BCD-Strep-PCaspase-R153A

   Plasmid

   #214036

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R153A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

   Plasmid

   #218426

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   5X: K2A, K13A, R15A, K20A, K23A mutations of both…

   Promoter

   CMV

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

   Plasmid

   #218425

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   5X: K2A, K13A, R15A, K20A, K23A mutations of down…

   Promoter

   CMV

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

   Plasmid

   #212107

   Purpose

   Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   5X: K2A, K13A, R15A, K20A, K23A mutations of upst…

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSCBE3-NG-HF1

   Plasmid

   #218160

   Purpose

   This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCBE3-NG-Hypa

   Plasmid

   #218161

   Purpose

   This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Tags

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N692A…

   Promoter

   rpsL promoter

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-BCL9

   Plasmid

   #202662

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of BCL9 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   BCL9 transcription coactivator (BCL9 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDM4_Deletion_Downstream_gRNA_2

    Plasmid

    #195137

    Purpose

    gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 Deletion Downstream gRNA 2 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MDM4_CRISPRi_gRNA_2

    Plasmid

    #195133

    Purpose

    gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 CRISPRi gRNA 2 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MDM4_CRISPRi_gRNA_1

    Plasmid

    #195132

    Purpose

    gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 CRISPRi gRNA 1 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MDM4_Deletion_Downstream_gRNA_1

    Plasmid

    #195136

    Purpose

    gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MDM4 Deletion Downstream gRNA 1 (MDM4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-R/G-ICUE

    Plasmid

    #181849

    Purpose

    ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    R/G-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

    Plasmid

    #170193

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    CMV51

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

    Plasmid

    #170194

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Use

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    CMV51

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

    Plasmid

    #170189

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    N501Y

    Promoter

    CMV51

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. SOD1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176964

    Purpose

    Mammalian expression vector encoding SOD1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-ZNF385A

    Plasmid

    #101615

    Purpose

    Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF385A (ZNF385A Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-FOXD4L3

    Plasmid

    #101491

    Purpose

    Donor Vector containing FOXD4L3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXD4L3 (FOXD4L3 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge