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Showing: 521 - 540 of 786 results

  1. pOD2048-csnDEG

    Plasmid
    #89368
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Posm-6
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  2. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89369-plasmid-map-sequence-id-177235

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  3. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  4. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Tags
    Expression
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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  14. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  15. pCB11

    Plasmid
    #201111
    Purpose
    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.
    Depositor
    Mary Dunlop
    Article
    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:
    Inserts
    CcaR
    tetA(C)
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) lea…
    Promoter
    J23100 and PcpcG2-172
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    201111-plasmid-map-sequence-id-403029

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  17. pTd-4-15-13-10

    Plasmid
    #63835
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pTd-4-15-13-1

    Plasmid
    #63826
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    63826-plasmid-map-sequence-id-106559

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  19. pTd-4-15-13-2

    Plasmid
    #63827
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pTd-4-15-13-3

    Plasmid
    #63828
    Purpose
    This plasmid is designed to validate the biophysical model of translational coupling
    Depositor
    Howard Salis
    Article
    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71
    Insert
    CFP, mRFP1 and GFPmut3b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The intergenic distance between each gene is -4 n…
    Promoter
    J23100
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 540 of 786 results