We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1(+)-AKAP95-FRB

   Plasmid

   #138254

   Purpose

   Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   AKAP95-FRB (AKAP8 Human)
   

   Use

   Tags

   FRB rapamycin binding domain and HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-3xFLAG-V5-ccdB

   Plasmid

   #87064

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-PKI-GFP

   Plasmid

   #118482

   Purpose

   GFP-tagged Protein Kinase A inhibitor peptide.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   PKI-GFP
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-PKAcat-mCherry

   Plasmid

   #181841

   Purpose

   mCherry-tagged PKA catalytic subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PKAcat-mCherry (PRKACA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-FluoSTEP-ICUE

   Plasmid

   #181845

   Purpose

   Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   FluoSTEP-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-FGFR1c-V5/HIS

   Plasmid

   #201106

   Purpose

   expression of human FGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 SARS-CoV-2 N

   Plasmid

   #158079

   Purpose

   Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 nucleocapsid (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaV2.1/pcDNA3.1 ~ Human

   Plasmid

   #140575

   Purpose

   Expression in mammallian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

   Insert

   CaV2.1 Human (CACNA1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaV1.2/pcDNA3.1~ Human

    Plasmid

    #140576

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    human CaV1.2 (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRP4-Flag/pCDNA3.1

    Plasmid

    #197291

    Purpose

    Plasmid expressing an antigen targeting LRP4 (extracellular)

    Depositor

    Steve Burden

    Insert

    LRP4 (Lrp4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-ERBB4-V5/HIS

    Plasmid

    #201105

    Purpose

    expression of human ERBB4 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-HA-FbxO11-pcDNA3.1-

    Plasmid

    #52501

    Purpose

    expresses human FbxO11 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-MS2-HA-HsNBAS_AA

    Plasmid

    #148236

    Purpose

    Mammalian Expression of HsNBAS

    Depositor

    Elisa Izaurralde

    Insert

    HsNBAS (NBAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

    Plasmid

    #187729

    Purpose

    Express the catalytically inactive NEDD4L FL in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    Cys874 mutated to a serine

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-FLAG-Claspin

    Plasmid

    #12659

    Depositor

    Michele Pagano

    Article

    Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-CHRNA7-mGFP

    Plasmid

    #62629

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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