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pJDC60

Plasmid

#157780

Purpose

tdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al Methods Mol Biol. 2018;1790:75-85. doi:

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

L5

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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V-Kras

Plasmid

#27806

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. . 13(3):031204.

Insert

Venus kras

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBS4

Plasmid

#83793

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Use

Tags

CFP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBdxs

Plasmid

#17821

Depositor

Jay Keasling

Article

Kim et al Biotechnol Bioeng. 2001 Feb 20. 72(4):40

Insert

dxs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pBES2

Plasmid

#83800

Purpose

Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

Venus

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pHK201

Plasmid

#235481

Purpose

Repression of gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII, cI repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK328

Plasmid

#235479

Purpose

Inducible gene VIII by Pbad/lac hybrid promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK318

Plasmid

#235478

Purpose

Inducible gene VIII by Plux/tet hybrid promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Ec FkP-dnaN(3E's)-pRSFDuet

Plasmid

#238344

Purpose

over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

Depositor

Michael O'Donnell

Insert

PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)

Use

Tags

3X FLAG+PKA kinase motif+PreScission Protease cle…

Expression

Bacterial

Mutation

R96E; R103E; R105E

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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Ec FkP-dnaN(3A's)-pRSFDuet

Plasmid

#238343

Purpose

over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

Depositor

Michael O'Donnell

Insert

PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)

Use

Tags

3X FLAG+PKA kinase motif+PreScission Protease cle…

Expression

Bacterial

Mutation

R96A; R103A; R105A

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Ec FkP-dnaN(3S's)-pRSFDuet

Plasmid

#238345

Purpose

over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

Depositor

Michael O'Donnell

Insert

PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)

Use

Tags

3X FLAG+PKA kinase motif+PreScission Protease cle…

Expression

Bacterial

Mutation

R96S; R103S; R105S

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK316

Plasmid

#235474

Purpose

Inducible gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK326

Plasmid

#235475

Purpose

Inducible gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK346

Plasmid

#235477

Purpose

Inducible gene VIII by Pbad promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK336

Plasmid

#235476

Purpose

Inducible gene VIII by Plux promoter

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

phage M13 geneVIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.2

Plasmid

#235450

Purpose

NOT gate receiver with bs for sgRNA2

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

NOT gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.3

Plasmid

#235451

Purpose

NOT gate receiver with bs for sgRNA3

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

NOT gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.4

Plasmid

#235452

Purpose

NOT gate receiver with bs for sgRNA4

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

NOT gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHK001.5

Plasmid

#235453

Purpose

NOT gate receiver with bs for sgRNA5

Depositor

Yolanda Schaerli

Article

et al Nat Commun 16, 3569 (2025)

Insert

NOT gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge