We narrowed to 1,204 results for: GR

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pcDNA3-FLAG3-drAgo2DY-EF

Plasmid

#221733

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E and Y680F in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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LCB1_v1.3 (MTK 3a) (pZYW044)

Plasmid

#194227

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB1

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB1_v1.3

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LCB3_v1.2 (MTK 3a) (pZYW045)

Plasmid

#194228

Purpose

Mammalian Toolkit part 3a encoding the synthetic Spike binder LCB3

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

LCB3_v1.2

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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WPRE (MTK 4b) (pAN2410)

Plasmid

#194235

Purpose

Mammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbones

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

WPRE terminator

Use

Synthetic Biology

Promoter

None

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-deLACCO

Plasmid

#167945

Purpose

Fluorescent biosensor control for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

deLACCO

Expression

Bacterial

Promoter

araBAD

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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MTK234_058

Plasmid

#123984

Purpose

Encodes the spCas9 Safetargeting Chr11 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sg Safetargeting Chr11

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_038

Plasmid

#123917

Purpose

Encodes the spCas9 pSV40 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_043

Plasmid

#123913

Purpose

Encodes the spCas9 pSV40 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA-pSV40

Expression

Mammalian

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_015

Plasmid

#123991

Purpose

Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hROSA26 sg4

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_005

Plasmid

#123885

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_021

Plasmid

#123936

Purpose

Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL3R4 spacer

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_047

Plasmid

#124055

Purpose

Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_048

Plasmid

#124056

Purpose

Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_049

Plasmid

#124057

Purpose

Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB340

Plasmid

#124023

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::iRFP713-bgPA_R1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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