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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-clbB

Plasmid

#114155

Purpose

Expresses full-length ClbB

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbB

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-mxLOX1

Plasmid

#104975

Purpose

Biosynthesis of oxylipins by microbial enzymes

Depositor

Deokkun Oh

Insert

MXAN_1745 (MXAN_1745 Myxococcus xanthus)

Use

Tags

6 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-mxLOX2

Plasmid

#104976

Purpose

Biosynthesis of oxylipins by microbial enzymes

Depositor

Deokkun Oh

Insert

MXAN_1744 (MXAN_1744 Myxococcus xanthus)

Use

Tags

6 histidine tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-MtrR

Plasmid

#51188

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

MtrR

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-JadR2

Plasmid

#51178

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

JadR2

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pET23a-MmUBC

Plasmid

#69555

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBC (Ubc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28GSTlinker-LIC

Plasmid

#119970

Purpose

Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Use

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pET21b-RL020A

Plasmid

#42138

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mVenus-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH17A

Plasmid

#86462

Purpose

Expression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH17A

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-Ava3856

Plasmid

#45742

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3856 (Ava_3856 Anabaena variabilis)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only