We narrowed to 165,075 results for: addgene

1. Golgi-FAST-IRES-iRFP670

   Plasmid

   #216345

   Purpose

   For testing localization of pFAST and iRFP670 proteins to Golgi complex

   Depositor

   Alison Tebo

   Insert

   ST6Gal1_pFAST-IRES-Sys1_iRFP670
   

   Tags

   myc for FAST and iRF670 proteins

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Peroxisomes-FAST-IRES-iRFP670

   Plasmid

   #216347

   Purpose

   For testing localization of pFAST and iRFP670 proteins to peroxisomes

   Depositor

   Alison Tebo

   Insert

   pFAST_SKL-IRES-iRFP670_PXMP2
   

   Tags

   myc for FAST protein only

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Mito-FAST-IRES-iRFP670

   Plasmid

   #216346

   Purpose

   For testing localization of pFAST and iRFP670 proteins to mitochondria

   Depositor

   Alison Tebo

   Insert

   Cox8_pFAST-IRES-Tomm20_iRFP670
   

   Tags

   myc for FAST and iRF670 proteins

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV V5-Senp2

   Plasmid

   #175118

   Purpose

   Lentiviral expression of V5-tagged mouse Senp2

   Depositor

   Larry Gerace

   Insert

   Senp2 (Senp2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-RB-PE2

   Plasmid

   #173902

   Purpose

   SB-transposon with constitutive expression of SpCas9 PE2

   Depositor

   Ronald Cohn

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6V41

   Plasmid

   #162272

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   CDY1 (CDY1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 6WAU

   Plasmid

   #162269

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   PHF19 (PHF19 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 6WAJ

   Plasmid

   #162267

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   NLE1 (NLE1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. 6OGN

   Plasmid

   #162260

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   PRMT7 (PRMT7 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAMTA2.1.0-gDNA

    Plasmid

    #132456

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CAMTA2 (CAMTA2 Human)
    

    Use

    CRISPR

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMBD1.1.0-gDNA

    Plasmid

    #132444

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    MBD1 (MBD1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6NIM

    Plasmid

    #127294

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD18
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 26, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 5VS7 (PP-BRD4)

    Plasmid

    #101264

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD4
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 5KO4 (PP-BRD19)

    Plasmid

    #101882

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD19
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. 5TCK (PP-BRD3)

    Plasmid

    #101887

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. 5U9N (PP-BRD3)

    Plasmid

    #101255

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. 4QNY

    Plasmid

    #61958

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LdBPK_331470.1:MAC04E-A10:C228347
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. PP-MAPK2 (3N9X)

    Plasmid

    #28117

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PBG-PF11_0147
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

19. PP-CDPK3

    Plasmid

    #28151

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDPK3
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    CAD domain (residues 425-602).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

20. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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