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Plasmid#126921PurposePlasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1DepositorInsertHMG1
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB19-PapRS#34-OmpA
Plasmid#231105PurposeVector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDUP3
Plasmid#92174PurposeVector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.DepositorInsertNAT1
UseGenbank: u03440.1TagsExpressionYeastMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-KIRIN1-GR
Plasmid#107417PurposeBacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GRDepositorInsertKIRIN1-GR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
BLING 1
Plasmid#171647PurposeExpresses bioluminescent glutamate indicator in mammalian cellsDepositorInsertBLING 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-irp3 WT
Plasmid#87106Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocoliticaDepositorInsertirp3 WT
UseTagshis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
GGE020
Plasmid#120777PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)DepositorInsertT1-3_Lip2
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE021
Plasmid#120778PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)DepositorInsertT2-3_Lip2
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSF- RPA4 (MSW#716)
Plasmid#208077PurposeExpression of human Replication Protein A 4 in E.coliDepositorInsertRPA4 (RPA4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO5
Plasmid#193749PurposeExpresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selectionDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO6
Plasmid#193747PurposeExpresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,DepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR
Plasmid#193746PurposeExpresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationK198E- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
UseTagsExpressionBacterialMutationG160S- please see depositor commentsPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B3.MJ0130m
Plasmid#11528DepositorInsertType I restriction-modification enzyme (MJ_0130m M. jannaschii)
UseTagsHisTag, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB_mU6_enh-gRNA_Puro-T2A-BFP
Plasmid#235571PurposeEnhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.DepositorInsertsgRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only