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pFosill-1

Plasmid

#89695

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNR188

Plasmid

#79543

Purpose

Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFF753

Plasmid

#61453

Purpose

IPTG-inducible msd(wt)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd(wt)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIKpBAD

Plasmid

#129273

Purpose

Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKM489-Snap

Plasmid

#108437

Purpose

ORBIT integrating plasmid for C-terminal tagging with SNAP tag.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-SNAP tag

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Expression

Yeast

Promoter

TEF

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRec

Plasmid

#104572

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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ECFP-Rab5C-S35N

Plasmid

#110504

Purpose

Dominant Negative

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab5C-S35N (RAB5C Human)

Tags

ECFP

Expression

Mammalian

Mutation

S35N

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFosill-3

Plasmid

#89697

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJW1507

Plasmid

#62361

Purpose

pFA6a -mVenus-BirA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

c-terminal mVenus-BirA tagging

Use

Yeast c-terminal tagging vector

Tags

mVenus-BirA

Mutation

L232H mutation compared to standard mVenus

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc- GFP-Flag

Plasmid

#82471

Purpose

Expresses myc-GFP-Flag

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-GFP-Flag

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAN3944

Plasmid

#74702

Purpose

Circuit plasmid: 0x1C

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x1C

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Dual-recombinase-controller

Plasmid

#44456

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

pTEt-Bxb1, pBAD-TP901

Use

Synthetic Biology

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pIF3

Plasmid

#196068

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Promoter

MoRP27 promoter

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pQacR-Q2

Plasmid

#74690

Purpose

QacR-Q2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ2-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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