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pSTTa

Plasmid

#248647

Purpose

E. coli protein expression vector encoding 2x N-terminal StrepII tags flanking an 11 residue flexible linker, thrombin and TEV protease sites, and an optional C-terminal FLAG tag.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Type

Empty backbone

Tags

2x StrepII, Thrombin site, TEV site and FLAG

Expression

Bacterial

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3_T20-mSGH-AKAR3EV

Plasmid

#236210

Purpose

Fluorescent biosensor for PKA activity proximal to mitochondria

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

Tomm20-QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mBJH-AKAR3EV

Plasmid

#236206

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mSGH-AKAR3EV

Plasmid

#236207

Purpose

Fluorescent biosensor for cytosolic PKA activity

Depositor

Peter Dedecker

Article

Van Thillo et al ACS Nano. 2025 Jul 1;19(25):22755-22766.

Insert

QC2-AKAR-Halotag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSS22-IRES-NLS-eGFP

Plasmid

#241837

Purpose

Part I of the Donor construct for UBE3A reporter

Depositor

Albert Keung

Article

Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

Insert

eGFP

Use

Donor plasmid

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-TdTomato-WPRE-polyA

Plasmid

#241828

Purpose

AAV construct for constitutive expression of TdTomato.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

TdTomato-WPRE-polyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GST-EGFP-GPIDAF

Plasmid

#213716

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

hPGK

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GST-EGFP-GPIDAF

Plasmid

#213722

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

V5-tag-GST

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GST-EGFP-GPIBY55

Plasmid

#213717

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

hPGK

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GST-EGFP-GPICEAM7

Plasmid

#213718

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an hPGK promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

GST

Expression

Mammalian

Promoter

hPGK

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GST-EGFP-GPICEAM7

Plasmid

#213723

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Tags

V5-tag-GST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TST-EGFP-GPIBY55

Plasmid

#213711

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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FB395

Plasmid

#203631

Purpose

TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1aG2b.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB396

Plasmid

#203632

Purpose

TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1ab.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB397

Plasmid

#203633

Purpose

TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1abc.3:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYI047

Plasmid

#170843

Purpose

(pFRO6::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pFRO6::SMT2-FLAG

Expression

Plant

Promoter

FRO6p

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI048

Plasmid

#170844

Purpose

(pGH3.17::SMT2-FLAG::tOCS)

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

pGH3.17::SMT2-FLAG

Expression

Plant

Promoter

GH3.17p

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYI012

Plasmid

#170841

Purpose

pGH3.17::GUSPlus::tOCS

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GH3.17p

Expression

Plant

Promoter

GH3.17p

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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