We narrowed to 24,275 results for: CHI

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pBiFC-HA-hCaspase 2 prodomain-VC155

Plasmid

#49261

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyB621-mCherry-HRasCT

Plasmid

#100282

Purpose

Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-621 aa) (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-621 aa)

Promoter

CAG promoter

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-HA-mBec1

Plasmid

#99505

Purpose

3rd generation lentiviral expression of wild-type Becn1

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

Plasmid

#182231

Purpose

Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member B (RHOB Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Promoter

TRE bidirectional

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

Plasmid

#87827

Purpose

Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 CARD-VC155

Plasmid

#49259

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

Caspase-2 CARD (CASP2 Human)

Tags

HA and split Venus C

Expression

Mammalian

Mutation

deleted aa 123-435

Promoter

CMV

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 FL (C303S)-VN173

Plasmid

#49258

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 (CASP2 Human)

Tags

flag and split Venus N

Expression

Mammalian

Mutation

C303S

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro

Plasmid

#192682

Purpose

Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNAs #1,2,3 (ASCL1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC591 - pCMV Mem-iRFP

Plasmid

#59134

Purpose

A plasmid that express membrane-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Membrane-localized Infrared Fluorescent Protein

Tags

Palmitylation signal

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-shD

Plasmid

#21964

Depositor

Lynda Chin

Article

Ji et al Nature. 2007 Aug 16. 448(7155):807-10.

Insert

si NEDD9 (NEDD9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only
pRS423-mitoT

Plasmid

#174525

Purpose

Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

Yeast mitochondrial intermembrane tether

Use

Synthetic Biology

Tags

eGFP

Expression

Yeast

Promoter

MET25

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 Nlgn2

Plasmid

#59358

Purpose

Lentiviral expression vector: Neuroligin 2 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

Neuroligin 2 shRNA (rat and mouse) (Nlgn2, Nlgn2 Rat, Mouse)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_NOPLight-ctr

Plasmid

#195579

Purpose

Expresses the control sensor NOPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight-ctr

Expression

Mammalian

Mutation

D110A, D130A

Promoter

CMV

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236A-pcDNA3.1

Plasmid

#85764

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236A

Promoter

CMV

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_hSynapsin1_NOPLight1

Plasmid

#195580

Purpose

Expresses NOPLight1 in neurons

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight1

Use

AAV

Expression

Mammalian

Promoter

human Synapsin-1

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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