We narrowed to 6,726 results for: SIM;

1. pLVX-NLS-HA-TurboID-PCNA

   Plasmid

   #215076

   Purpose

   Expresses NLS-HA-TurboID-PCNA in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   NLS-HA-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVpuro-eGFP-mcGAS

   Plasmid

   #108675

   Purpose

   For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   eGFP-cGAS (Cgas Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BM00654

   Plasmid

   #155339

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Nsp15

   Plasmid

   #156481

   Purpose

   Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-FuG-E

   Plasmid

   #67509

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

   Insert

   Fusion Glycoprotein type E
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM33_Nsp5_Mpro

   Plasmid

   #156475

   Purpose

   Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

   Plasmid

   #107311

   Purpose

   Encoding scFv-p65-HSF1 driven by EF1α promoter

   Depositor

   Hui Yang

   Article

   Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

   Insert

   EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_Nsp10

   Plasmid

   #156479

   Purpose

   Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AcGFP-MSH2

    Plasmid

    #215121

    Purpose

    Expresses AcGFP-MSH2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH2 (MSH2 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROS17

    Plasmid

    #107931

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-GAL4-IL10-IRES-mCherry-PGK-BFP

    Plasmid

    #223209

    Purpose

    Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL10 (IL10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_Nsp4

    Plasmid

    #156474

    Purpose

    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA6.2-YFP-CWT

    Plasmid

    #176689

    Purpose

    Fluorescent reporter for phagosomal escape of Staphylococcus aureus

    Depositor

    Martin Fraunholz

    Article

    Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

    Insert

    cell wall-targeting domain of Lysostaphin
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    deletion of protase domain of Lysostaphin

    Promoter

    CMV

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBKWH-p53

    Plasmid

    #133900

    Purpose

    Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    p53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

    Plasmid

    #223213

    Purpose

    Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    TGFb1 active - membrane (TGFB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

    Plasmid

    #107313

    Purpose

    Encoding aCamkII-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)

    Plasmid

    #208395

    Purpose

    To generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    Human MRE11 (MRE11 Human)
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DB1delta/DB2delta

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAPTrap-mtagBFP2-IRESMuro

    Plasmid

    #82335

    Purpose

    Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    mtagBFP2-IRESMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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