We narrowed to 10,769 results for: ESP

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pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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EZH2 E10.3 gRNA

Plasmid

#90685

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302-DUSP19-V5 puro

Plasmid

#87772

Purpose

Expresses V5 tagged human DUSP19

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

DUSP19 (DUSP19 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1 Y357F

Plasmid

#18882

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 Y357F (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y357F

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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CBX3 A6.5 gRNA

Plasmid

#90569

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX3 (Guide Designation A6.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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CBX3 A5.5 gRNA

Plasmid

#90568

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX3 (Guide Designation A5.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(ala)

Plasmid

#15314

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(ala) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Ala Thr-224Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
RRM1 E2.3 gRNA

Plasmid

#90881

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM1 (Guide Designation E2.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100

Plasmid

#19848

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1-N100 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

deleted amino acids 1-100

Available Since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
3xFLAG-dCas9/pMXs-IG

Plasmid

#51258

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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RRM2 E4.3 gRNA

Plasmid

#90883

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM2 (Guide Designation E4.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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RRM2 E3.3 gRNA

Plasmid

#90882

Purpose

3rd generation lentiviral gRNA plasmid targeting human RRM2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

RRM2 (Guide Designation E3.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAG70 BDNF 3'UTR

Plasmid

#12021

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

BDNF 3'UTR (BDNF Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-RBP-LOV2

Plasmid

#185792

Purpose

Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

Depositor

Lizhong He

Article

Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

Insert

RBP-LOV2

Tags

10xHis, TEV protease cleavage site after His Tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-GFP-RGG

Plasmid

#124937

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C2518

Plasmid

#91087

Purpose

Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

TaU6

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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