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pBad_crtEBI_37

Plasmid

#63807

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1

Plasmid

#91942

Purpose

Bacterial expression of maize SAE1

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Insert

HA-ZmSAE1

Tags

HA

Expression

Bacterial

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBXNAC3H

Plasmid

#47074

Purpose

FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGC163

Plasmid

#19635

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC225

Plasmid

#19636

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pBbA3c-RFP

Plasmid

#35308

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pBbE4c-RFP

Plasmid

#35296

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProE

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGC160

Plasmid

#19632

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCTK132

Plasmid

#248495

Purpose

Encodes PTet-RiboJ-B0064 as a Type 2 part to be used in the CTK/YTK systems

Depositor

Heinz Koeppl

Article

Mejlsted et al ACS Synth Biol. 2026 Jan 25. doi: 10.102

Insert

PTet-RiboJ-B0064

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pNO201

Plasmid

#249344

Purpose

Targeted gene disruption by use of a group II intron (targetron) vector in Clostridia

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Insert

group II intron and ItrA

Use

E. coli - clostridium shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK125

Plasmid

#245147

Purpose

pJAK081 carrying Pcwp2-mScarlet-I

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Pcwp2-mScarlet-I

Use

Bacterial knock-in

Promoter

Pcwp2

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK201

Plasmid

#245149

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 1 (AAGTCCTCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK202

Plasmid

#245150

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 2 (TCTTGACCG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK203

Plasmid

#245151

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 3 (AACAACACC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK204

Plasmid

#245152

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 4 (AACAGGTGG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK205

Plasmid

#245153

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 5 (ACCGATTAG)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK206

Plasmid

#245154

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 6 (CACTTATGC)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pJAK207

Plasmid

#245155

Purpose

pJAK081-based knock-in plasmid with a 218 bp insertion containing 9 bp sequencing Barcode 7 (CCTCCAACT)

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Type

Empty backbone

Use

Bacterial knock-in

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge