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pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Clover-MAPTau-C-10

Plasmid

#56371

Purpose

Localization: Microtubules, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MAPTau (MAPT Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHIVT9-NAT-3xFlag-SMARCAL1-ΔN

Plasmid

#246936

Purpose

Lentiviral vector that expresses Flag-tagged SMARCAL1 with a deletion of the RPA-binding domain located in the N-terminal region (ΔN)

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHIVT9-NAT-3xFlag-SMARCAL1-R764Q

Plasmid

#246937

Purpose

Lentiviral vector that expresses Flag-tagged SMARCAL1 with a mutation in the ATP translocase domain (R764Q)

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHIVT9-NAT-3xFlag-SMARCAL1

Plasmid

#246935

Purpose

Lentiviral vector that expresses wildtype Flag-tagged SMARCAL1

Depositor

Daniel Durocher

Article

Feng et al Mol Cell. 2024 Dec 5;84(23):4522-4537.e7

Insert

SMARCAL1 (SMARCAL1 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

EF-1-alpha promoter

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-huCof R21Q-mRFP

Plasmid

#245964

Purpose

Expression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

R21Q

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huCof K22Q-mRFP

Plasmid

#245965

Purpose

Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-eGFP

Plasmid

#179924

Purpose

C terminal fusion of EGFP to mouse HP1α (Cbx5)

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-EGFP

Plasmid

#179926

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3)

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hIRF5-V5(4D)-LPETG

Plasmid

#237225

Purpose

Expresses LPETG-tagged, constitutively active human IRF5 variant in bacteria

Depositor

Elliot Chaikof

Article

Eweje et al Nat Biotechnol. 2025 May 15. doi: 10.103

Insert

Interferon regulatory factor 5 (IRF5 Human)

Tags

6xHis

Expression

Bacterial

Mutation

S451D, S453D, S456D, S462D

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-Positron2-p10

Plasmid

#239082

Purpose

Gal4 activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Insect

Mutation

R78K N81D D92N W178F

Promoter

hsp70

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10

Plasmid

#239081

Purpose

LexA activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Eric R. et al Janelia Figshare 2023

Insert

Positron2

Expression

Insect

Mutation

R78K N81D D92N W178F

Promoter

hsp70

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-LG4_chr5:550kb antisense

Plasmid

#205468

Purpose

Enhancer containing plasmid used for assessing enhancer::promoter interactions

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

chr5:550kb LG4

Use

Synthetic Biology; Subcloning of taq-amplified pc…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Ce iPGM-C-HiBiT

Plasmid

#207120

Purpose

Bacterially expressed iPGM-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)

Tags

HiBiT Tag and Thrombin cleavage site + 10x His Ta…

Expression

Bacterial

Mutation

Codon optmization for expression in BL21DE3

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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E. coli Lig-N-HiBiT

Plasmid

#215437

Purpose

Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin

Tags

10xHis-tag with thrombin site and HiBiT Tag

Expression

Bacterial

Mutation

Codon Optimized for bacterial expression, T7 gene…

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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E. coli Lig-C-HiBiT

Plasmid

#215438

Purpose

Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin

Tags

10xHis-tag with thrombin site and HiBiT Tag

Expression

Bacterial

Mutation

Codon Optimized for bacterial expression, T7 gene…

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-TDP43HP

Plasmid

#237669

Purpose

For overexpression of mEGFP-NPM1-TDP43HP

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-TDP43HP (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1

Plasmid

#237661

Purpose

For integration of EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1 (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS

Plasmid

#237662

Purpose

For integration of EGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-FtoA

Plasmid

#237664

Purpose

For integration of EGFP-NPM1-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoA (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-0F

Plasmid

#237665

Purpose

For integration of EGFP-NPM1-KS-0F

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-0F (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-KS

Plasmid

#237668

Purpose

For overexpression of mEGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_TetON_EGFP-NPM1-KS-FtoG

Plasmid

#237663

Purpose

For integration of EGFP-NPM1-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-FtoG (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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mCGN-Myc-His

Plasmid

#236510

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Tags

Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-Myc

Plasmid

#236509

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1-Myc

Plasmid

#236514

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGNL1 (Cgnl1 Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d sgCiPELO #1

Plasmid

#228932

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA355_sgCiCh2-2

Plasmid

#229021

Purpose

Expression of a CRISPRi doxycycline inducible control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_023d sgCiPELO #8

Plasmid

#228934

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221112

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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