We narrowed to 9,149 results for: Pol

1. pBa.GFP-AP1/beta1

   Plasmid

   #218802

   Purpose

   Mammalian expression o fhuman AP1 b1

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   AP1 b1 (AP1B1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

   Plasmid

   #172226

   Purpose

   Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposon

   Tags

   3XFLAG, DD-domain, GS-linker, and TagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-IBBN (G2)

   Plasmid

   #85500

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. A3B-Cas9n-UGI-NLS

   Plasmid

   #198889

   Purpose

   Express full-length A3B BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. -227 hTF (mutant NF-kB)

   Plasmid

   #15443

   Depositor

   Nigel Mackman

   Article

   Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

   Insert

   Tissue factor promoter (F3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   NF-kappaB binding domain mutated

   Available Since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

6. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. psicheck-2-Casp8

   Plasmid

   #48172

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of CASP8 (CASP8 Human)
   

   Use

   Luciferase

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. sg1+sg2+sg3

   Plasmid

   #113969

   Purpose

   Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1+sg2+sg3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV Puro SCRIB P305L

   Plasmid

   #88887

   Purpose

   SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Mutation

   Proline to Leucine mutation @ 305th amino acid

   Promoter

   MSCV promoter

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. hsp70:Stathmin1-mKate2

    Plasmid

    #105969

    Purpose

    heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Stathmin1 (STMN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Hu_Plk5(WT)

    Plasmid

    #136319

    Purpose

    Mammalian expression of human Plk5-WT with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-Enhancer-ZC3H12D_PromoterWT

    Plasmid

    #153066

    Purpose

    ZC3H12D promoter region cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region (ZC3H12D Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    none

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1 FRB-myc-RagA

    Plasmid

    #112740

    Purpose

    express FRB-myc-RagA

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagA (RRAGA Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPA-fapO-RgTALsyn

    Plasmid

    #100946

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    GAP (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-Strep/HA-UBE2QL1

    Plasmid

    #124665

    Purpose

    Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 FRB-myc-RagC

    Plasmid

    #112741

    Purpose

    express FRB-myc-RagC

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    FRB-myc-RagC (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    FRB, myc

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-hspB10

    Plasmid

    #63101

    Purpose

    Expression of HSPB10 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-6P1-Ataxin3Q22-C14A

    Plasmid

    #22113

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C14A

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only