We narrowed to 12,952 results for: sequence

1. pSirenRetroQ(puro)-shEgr4

   Plasmid

   #108117

   Purpose

   pSIREN-RetroQ vector containing shRNA sequence to Egr4

   Depositor

   Peter Johnson

   Article

   Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

   Insert

   shRNA to Egr4 (Egr4 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL704

   Plasmid

   #140374

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-4BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJBL705

   Plasmid

   #140375

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-5BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTL-Bag3-gRNA3

   Plasmid

   #196254

   Purpose

   Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTL-Bag2-gRNA2

   Plasmid

   #196252

   Purpose

   Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTL-Bag1-gRNA1

   Plasmid

   #196251

   Purpose

   Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTL-Bag4-gRNA4

   Plasmid

   #196255

   Purpose

   Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 375 pME-fli1a

   Plasmid

   #49001

   Purpose

   full length coding sequence for zebrafish fli1a gene in gateway middle entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   friend leukemia integration 1a (fli1 Zebrafish)
   

   Use

   Gateway middle entry

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV4-Flag-mIL-17RA/YFP.Hygro

   Plasmid

   #46862

   Purpose

   encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA-YFP
   

   Use

   Tags

   Flag/IL-3 signal sequence and fused to CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-N1-2x

    Plasmid

    #172282

    Purpose

    Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-2x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1-4x

    Plasmid

    #172283

    Purpose

    Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-4x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ-DoxOn-shCUX1-5150

    Plasmid

    #90469

    Purpose

    Lentiviral vector expressing a doxycycline-inducible shRNA against human CUX1 sequence starting at 5150 of M74099

    Depositor

    Alain Nepveu

    Article

    Ramdzan et al PLoS Biol. 2014 Mar 11;12(3):e1001807. d

    Insert

    TGCTGTTGACAGTGAGCGTCAGAGCGATAATACACTATTATAGTGAAGCC
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY71-tet.I.8

    Plasmid

    #133521

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 11 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-OsMIR390-B/c

    Plasmid

    #61468

    Purpose

    Gateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKT3Ts-goldyTALEN

    Plasmid

    #80330

    Purpose

    Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

    Depositor

    Jeffrey Essner

    Article

    Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-rev_U6_MCS

    Plasmid

    #109769

    Purpose

    Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    U6-gRNA cassette
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47450

    Purpose

    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5HD_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. D5_Ld_280600

    Plasmid

    #135821

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    major surface protease gp63, putative,leishmanolysin, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETa-mKikGR-V69T

    Plasmid

    #98574

    Purpose

    photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mKikGR-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PF3D7_0606800-bio

    Plasmid

    #47790

    Purpose

    Expresses enzymatically monobiotinylated full-length PF3D7_0606800 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PF3D7_0606800
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. T7T3-PacI-elfn1_459to1199

    Plasmid

    #87671

    Purpose

    In situ probe for mouse Elfn1

    Depositor

    Oscar Marin

    Article

    Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

    Insert

    Elfn1 (Elfn1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    partial sequence, (459 to 1199bp off NM_175522.3)

    Promoter

    T7/T3

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRpr150-LUC

    Plasmid

    #85700

    Purpose

    luciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    RpR 150 enhancer and hsp70 minimal promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    RpR 150 enhancer and hsp70 minimal promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

23. pT7-EGFP-C1-HsDCP2-F404AF407AF409A_V

    Plasmid

    #147813

    Purpose

    Mammalian Expression of HsDCP2-F404AF407AF409A

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP2-F404AF407AF409A (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sema4g-AP-His

    Plasmid

    #72034

    Purpose

    Expresses the extracellular region of the Sema4G protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4g (Sema4g Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Promoter

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47452

    Purpose

    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NI_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJBL729

    Plasmid

    #140399

    Purpose

    Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-cadO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47446

    Purpose

    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5HD_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS315-48nt-telo-I-SceI

    Plasmid

    #67623

    Purpose

    telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    3' gal1 unique sequence
    ura4 (ura4 Fission Yeast)
    48 bp telomere repeats
    hph
    

    Use

    Yeast cen/ars plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47445

    Purpose

    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NN_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge