We narrowed to 22,195 results for: his

1. pDDGFP-Leu2d-GgKDELR2 H12A

   Plasmid

   #123619

   Purpose

   Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   His12Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDDGFP-Leu2d-GgKDELR2 R47A

   Plasmid

   #123620

   Purpose

   Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg47Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDDGFP-Leu2d-GgKDELR2 R47K

   Plasmid

   #123621

   Purpose

   Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg47Lys

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDDGFP-Leu2d-GgKDELR2 E127A

   Plasmid

   #123622

   Purpose

   Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDDGFP-Leu2d-GgKDELR2 E127Q

   Plasmid

   #123623

   Purpose

   Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Gln

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDDGFP-Leu2d-GgKDELR2 Y158F

   Plasmid

   #123624

   Purpose

   Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Tyr158Phe

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDDGFP-Leu2d-GgKDELR2 R159A

   Plasmid

   #123625

   Purpose

   Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg159Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHTSHP-ShhN-Cys

   Plasmid

   #121136

   Purpose

   Expresses ShhN with a C-terminal cysteine residues in bacteria

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   shh (Shh Mouse)
   

   Use

   Tags

   HIS, HRV3C site, and MBP

   Expression

   Bacterial

   Mutation

   Amino acid sequence from 26-191, with an addition…

   Promoter

   tac

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hHSF1 S307A

   Plasmid

   #118364

   Purpose

   This plasmid expresses human HSF1 S307A mutant.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S307A

   Promoter

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Plasmid

    #101207

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A and Q695A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

    Plasmid

    #101201

    Purpose

    Bacterial expression plasmid for SpCas9, REC3 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C and S960C

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid

    #101214

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C and K855A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

    Plasmid

    #101200

    Purpose

    Bacterial expression plasmid for SpCas9, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C and D273C

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pet151D-TOPO-BROX 1-401

    Plasmid

    #89866

    Purpose

    Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    BROX (BROX Human)
    

    Use

    Tags

    His Tag, V5 tag

    Expression

    Bacterial

    Mutation

    expresses only residues 1-401

    Promoter

    T7

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. csd2_140-251/pET21a(+)

    Plasmid

    #83294

    Purpose

    csd2, construct:140-251, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-139 and 252-308

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. csd1_54-312/pET21a(+)

    Plasmid

    #83291

    Purpose

    csd1, construct: 54-312, pET21a(+) C-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-53

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

    Plasmid

    #83462

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, W4107A

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

    Plasmid

    #83460

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4303, S4144D

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

    Plasmid

    #83463

    Purpose

    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    CD16.7

    Expression

    Mammalian

    Mutation

    Amino-acids 4078-4169

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA4/TO h-PKD1 FLS L4137P-GST

    Plasmid

    #83455

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, L4137P

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA4/TO h-PKD1 FLS R4150C-GST

    Plasmid

    #83454

    Purpose

    Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    Polycystin-1 (PKD1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Amino-acids 4107-4303, R4150C

    Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. csd2_121-308/pET28b(+)

    Plasmid

    #83292

    Purpose

    csd2, construct: 121-308, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1544
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-120

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. csd1_125-312/pET28b(+)

    Plasmid

    #83290

    Purpose

    csd1, construct: 125-312, pET28b(+) NC-tag

    Depositor

    SeWon Suh

    Article

    An et al PLoS One. 2016 Oct 6;11(10):e0164243. do

    Insert

    hp1543
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acid 1-124

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNC2

    Plasmid

    #72438

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg

    Promoter

    T7 and n/a

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-22HT-MSI1(7-192)Q30D

    Plasmid

    #60346

    Purpose

    Q30D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    Q30D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Dec. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET-22HT-MSI1(7-192)R53E

    Plasmid

    #60352

    Purpose

    R53E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pET-22HT-MSI1(7-192)G64A

    Plasmid

    #60351

    Purpose

    G64A mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G64A; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pET-22HT-MSI1(7-192)H83L

    Plasmid

    #60350

    Purpose

    H83L mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83L; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET-22HT-MSI1(7-192)G35D

    Plasmid

    #60347

    Purpose

    G35D mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35D; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pET-22HT-MSI1(7-192)G35E

    Plasmid

    #60348

    Purpose

    G35E mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    G35E; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pET-22HT-MSI1(7-192)H83F

    Plasmid

    #60349

    Purpose

    H83F mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    H83F; contains amino acids 7-192 of Msi1

    Promoter

    T7

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pQTEV-C6orf60

    Plasmid

    #34813

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    C6orf60 (FAM184A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BRCA1-(219-408)

    Plasmid

    #35067

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-408

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

34. IFI16-(192-393)-HinA-E222A-E272A-E381A

    Plasmid

    #35061

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(192-393)-HinA (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Mutation

    Contains HinA domain: aa 192-393; E222A, E272A, a…

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pJ158

    Plasmid

    #29492

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pJ177

    Plasmid

    #29494

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pJ178

    Plasmid

    #29495

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pJ138

    Plasmid

    #29488

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Use

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Promoter

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

39. Tpa1(273-644)

    Plasmid

    #23141

    Depositor

    SeWon Suh

    Article

    Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

    Insert

    Tpa1 C-terminal domain (TPA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-272

    Promoter

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

40. BacPAK Myc-Adrm1

    Plasmid

    #19420

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only