We narrowed to 57,257 results for: %s

1. pmCherry-N1-EXO1b-D173A

   Plasmid

   #111622

   Purpose

   Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

   Depositor

   Marco Muzi-Falconi

   Article

   Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

   Insert

   Exonuclease 1b D173A (EXO1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG109 H2B-FAST

   Plasmid

   #130722

   Purpose

   Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   H2B-FAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

   Plasmid

   #178851

   Purpose

   Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L/L254A

   Promoter

   CAG

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

   Plasmid

   #145770

   Purpose

   expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR v2 puro hGSDME gRNA2

   Plasmid

   #223520

   Purpose

   Knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR-Flag-IKKalpha-KM

   Plasmid

   #15468

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase alpha-KM (Chuk Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Lysine at 44 to Alanine

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

7. P-rTetO-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170325

   Purpose

   Tetracycline-inducible expression of V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170328

   Purpose

   Double-floxed inverse Orientation V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. P-Ef1a-mCherry-T2A-RPL22-1xV5

   Plasmid

   #170324

   Purpose

   Expresses V5 and mCherry tagged RPL22

   Depositor

   Hemali Phatnani

   Article

   Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

   Insert

   RPL22 (RPL22 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR-HA-IKKalpha

    Plasmid

    #15469

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase alpha (Chuk Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCR-Flag-IKKbeta-KM

    Plasmid

    #15466

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase beta-KM (Ikbkb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Lysine at 44 to Alanine

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

12. DHX58

    Plasmid

    #155542

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DHX58 (DHX58 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GLE1

    Plasmid

    #155625

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GLE1 (GLE1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CLK2

    Plasmid

    #155459

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLK2 (CLK2 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRL838

    Plasmid

    #70680

    Purpose

    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

    Depositor

    Peter Wolk

    Article

    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. DHX37

    Plasmid

    #155539

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DHX37 (DHX37 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FIP1L1

    Plasmid

    #155605

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    FIP1L1 (FIP1L1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Use

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Mutation

    Promoter

    Tef1

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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