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pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

Plasmid

#221832

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1AR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221833

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

Plasmid

#221835

Purpose

Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

2xFLAG with dSERT homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

Plasmid

#221837

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT1BR homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

Plasmid

#221838

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

Plasmid

#221839

Purpose

Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

Depositor

Quentin Gaudry

Article

Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

Insert

7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms

Use

CRISPR

Expression

Insect and Mammalian

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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GPR89B_full_pGCS1

Plasmid

#238499

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

GPR89B (GPR89B Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pOP823

Plasmid

#235718

Purpose

Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

RAB5A (RAB5A Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Q79L, C19S, C63S, aa 1-212

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK2(delta MTS)-3xFLAG

Plasmid

#233896

Purpose

lentiviral overexpression vector for NADK2 (delta MTS)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK2 (NADK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

delta MTS (see Depositor Comments)

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgNADK2

Plasmid

#233897

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting NADK2

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting NADK2 (NADK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1645

Plasmid

#235719

Purpose

Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

p40phox PX domain (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

Cys at 2, C85S, aa 2-149

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK033 [Origin_repABC-pSymB_PartType3]

Plasmid

#229270

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_repABC-pSymB_PartType3

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

repABC-pSymB Origin

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK034 [Origin_repABC-pSymA_PartType3]

Plasmid

#229271

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_repABC-pSymA_PartType3

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

repABC-pSymA Origin

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-GFPenhancer

Plasmid

#202468

Purpose

Expresses the nanobody GFP enhancer after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP enhancer

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-GFPminimizer

Plasmid

#202469

Purpose

Expresses the nanobody GFP minimizer after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3481 MTK3-mCherry

Plasmid

#226672

Purpose

MTK3 part containing the coding sequence of mCherry.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

Removed BbsI sites

Promoter

none

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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