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pZac2.1-GfaABC1D-Ezrin T567D-BioID2-HA

Plasmid

#227682

Purpose

To over express Phospho-mimetic Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567D

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB-MeCP2

Plasmid

#110824

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RAPpGEX

Plasmid

#162241

Purpose

Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

Insert

lrpap1 (Lrpap1 Mouse)

Tags

GST-tag

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMEL13

Plasmid

#107919

Purpose

kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pROS10

Plasmid

#107924

Purpose

URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
EGFP-Tau(T217E)

Plasmid

#187024

Purpose

Expresses EGFP tagged human 2N4R tau (with T217E mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Glutamic acid

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-dCas9-VPR

Plasmid

#63801

Purpose

pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Yeast

Promoter

GPD

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(T217A)

Plasmid

#187025

Purpose

Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Alanine

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF208

Plasmid

#101549

Purpose

Donor Vector containing ZNF208 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF208 (ZNF208 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

Plasmid

#162576

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB
EcMutLE32K

Expression

Bacterial

Mutation

E32K mutated to give dominant negative phenotype

Promoter

pBAD

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-HOXA10

Plasmid

#97042

Purpose

Expresses HOXA10 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A10 (HOXA10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only
pOD2046-bwmDEG

Plasmid

#89367

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pmyo-3

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB-HaloDA1.0-IRES-EGFP-CAAX

Plasmid

#234410

Purpose

Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pOD2048-csnDEG

Plasmid

#89368

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Posm-6

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only
pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pVAX1-BMP2/7 +

Plasmid

#137911

Purpose

Constitutive mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, tandem)

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Inserts

BMP2 (BMP2 Human)
BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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