We narrowed to 6,088 results for: pcDNA 3.1

1. FLAG-hIes2

   Plasmid

   #15355

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   PAP-1 binding protein (INO80B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCD4-TagRFP-T (RHPMP151)

   Plasmid

   #119238

   Purpose

   Expresses CD4 fused to TagRFP-T

   Depositor

   Ricardo Henriques

   Article

   Pereira et al Front Immunol. 2019 Apr 5;10:675. doi: 1

   Insert

   CD4 (CD4 Human)
   

   Tags

   tagRFP-T

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CaMKIV-dCT

   Plasmid

   #126422

   Purpose

   Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PH Akt-Venus

   Plasmid

   #85223

   Purpose

   Venus tagged PH domain of Akt for use as a PIP3 sensor

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

   Insert

   PH AKT(1-148) (AKT1 Human)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HiBiT-FZD5

   Plasmid

   #195843

   Purpose

   Expresses human FZD5 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD5 (FZD5 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Opto-GPR55

   Plasmid

   #106026

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR55; Opto-GPR55; C4)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR55 (GPR55 Bovine, Human)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

   Plasmid

   #202760

   Purpose

   Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   huAsCpf1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HiBiT-FZD3

   Plasmid

   #195841

   Purpose

   Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD3 (FZD3 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   I292V and V495I

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTE2690

   Plasmid

   #137717

   Purpose

   Expresses dimpLbCas12a-SunTag in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dimpLbCas12a
   

   Use

   CRISPR

   Tags

   NLSx3 SV40 and SunTagx10

   Expression

   Mammalian

   Mutation

   D156R, G532R, K538V, Y542R, K595R, D832A,

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB07 (CK2beta)

    Plasmid

    #27091

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

12. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Arrb1-Q394L

    Plasmid

    #35402

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q394L

    Promoter

    CMV

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

14. Nuc-mTurq2-Apollo-NADP+

    Plasmid

    #193302

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    SV40 NLS and mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3s-ABE7.10

    Plasmid

    #113128

    Purpose

    expressing ABE7.10 (TadA-TadA*7.10-nCas9-NLS-HA), human codon optimized

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE 7.10
    

    Tags

    HA epitope and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF1 K298R

    Plasmid

    #118362

    Purpose

    This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K298R

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. Opto-GPR4

    Plasmid

    #106001

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR4 (GPR4 Bovine, Human)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge