3,636 results

1. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159677

   Purpose

   Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAW-GFP-2xmiR-34 (pAW-AS-25)

   Plasmid

   #40833

   Purpose

   miRNA sensor used in Drosophila cell lines

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   Two target sites perfectly complementary to miR-34
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMT-GFP-Pat coiled-coil (cc)

   Plasmid

   #31757

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Contains only the coiled-coil domain: aa 535-1457

   Promoter

   Metallothionein

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pENTR-GFP-Patronin CC+CKK

   Plasmid

   #59048

   Purpose

   for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-GFP-Patronin CC 534–1457

   Plasmid

   #59050

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. KHBD00638

   Plasmid

   #39705

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG3090 (Sox14 Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00474

   Plasmid

   #39584

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG17077 (pnt Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. KHBD00118

   Plasmid

   #34272

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG7345 (Sox21a Fly)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. dmBACCS2VL-IRES-dOrai-IRES-GFP

    Plasmid

    #72897

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KHBD00340

    Plasmid

    #34463

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8573 (su(Hw) Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pUASP dsRed Rab7 CA

    Plasmid

    #44825

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Khc73-mCherry

    Plasmid

    #31752

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Khc73 (Khc-73 Fly)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pUASp YFP Rho1 DN

    Plasmid

    #52231

    Purpose

    Drosophila expression of YFP-Rho1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rho1 (Rho1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T19N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pGEM-T Dmel Or85d

    Plasmid

    #59685

    Purpose

    Probe for Drosophila melanogaster Or85d expression

    Depositor

    Leslie Vosshall, Richard Axel

    Article

    Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

    Insert

    Or85d (Or85d Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pFOSGE_COF2G09

    Plasmid

    #70923

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    E(z) (E(z) Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. KHBD00732

    Plasmid

    #39749

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3848 (trr Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

18. OA-1050S (UASt-dCasRx)

    Plasmid

    #132419

    Purpose

    Express dCasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UASt

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFOSGE_COF2G08

    Plasmid

    #70915

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Parg (Parg Fly)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pTP10

    Plasmid

    #165797

    Purpose

    Drosophila TP10 poly(a) terminator element

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    TP10
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge