Addgene

We narrowed to 1,160 results for: GR

Showing: 811 - 840 of 1160 results

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pBPK1520-HEK3-CTTins-ng

Plasmid

#180019

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U7

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A30P-ng

Plasmid

#180018

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A30P (SNCA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.1

Plasmid

#208020

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Expression

Mutation

mouse-human fusion

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2-sgACSL4 clone2

Plasmid

#162129

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone1

Plasmid

#162128

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR v2-sgACSL4 clone3

Plasmid

#162130

Purpose

Lentiviral sgRNA plasmid targeting human ACSL4

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgACSL4 (ACSL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn S87A

Plasmid

#36058

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87A (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

S87A

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pYO1031

Plasmid

#235706

Purpose

Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
UVRAG (UVRAG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1101

Plasmid

#235708

Purpose

Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Inserts

Beclin 1 (BECN1 Human)
ATG14L (ATG14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Use

Tags

His6(TEV)

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP796

Plasmid

#235701

Purpose

Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 MIT domain (NRBF2 Human)

Use

Tags

His6(TEV)

Expression

Bacterial

Mutation

aa 1-89

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

Plasmid

#222854

Purpose

Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Use

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

Plasmid

#194226

Purpose

Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

Plasmid

#194233

Purpose

Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-R-iLACCO1.2

Plasmid

#208021

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-R-diLACCO1

Plasmid

#208022

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-diLACCO1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-deLACCO1

Plasmid

#208018

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-R-iLACCO1

Plasmid

#208019

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone2

Plasmid

#162123

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2-sgAMPK alpha2 clone3

Plasmid

#162124

Purpose

Lentiviral sgRNA plasmid targeting human AMPK alpha2

Depositor

Boyi Gan

Article

Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

Insert

sgAMPK alpha2 (PRKAA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
RalA (G23V)(mature peptide)-pcw107-V5

Plasmid

#64643

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
RalA (G23V)(mature peptide)-pcw107

Plasmid

#64642

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

V617F

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-HA-eLACCO2.1-NGR

Plasmid

#208096

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet, Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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