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pED14xQ-1

Plasmid

#134794

Purpose

IPTG inducible expression of Q-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Q-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xR-1

Plasmid

#134797

Purpose

IPTG inducible expression of R-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

R-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNK132d

Plasmid

#156370

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pNK141l2

Plasmid

#156385

Purpose

Expresses orthogonal RBS LSS-mKate2 reporter

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

LSS-mKate2

Use

Synthetic Biology

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNK131d

Plasmid

#156369

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK108h

Plasmid

#156368

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK083d

Plasmid

#156367

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184h

Plasmid

#156365

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184g

Plasmid

#156364

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184f2

Plasmid

#156363

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184e2

Plasmid

#156362

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184d3

Plasmid

#156361

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184c3

Plasmid

#156360

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB184b3

Plasmid

#156359

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAB172a

Plasmid

#156324

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pNK045p

Plasmid

#156335

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXCA3H

Plasmid

#47072

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pED17x11

Plasmid

#134814

Purpose

sfGFP expression plasmid for characterizing qtRNA charging

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

sfGFP-151-TAGA

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

151-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pED14xS-4

Plasmid

#134802

Purpose

IPTG inducible expression of S-TAGA-Evo3 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA-Evo3 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED14xY-3

Plasmid

#134808

Purpose

IPTG inducible expression of Y-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA-Evo1 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAM9

Plasmid

#78560

Purpose

pBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.

Depositor

James Kaper

Article

Merrell et al J Bacteriol. 2001 May;183(9):2746-54.

Insert

ompU

Expression

Bacterial

Promoter

araBAD promoter

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pRF032d

Plasmid

#156353

Purpose

Expresses heterologous rRNA

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

rRNA operon, orthogonal-antiRBS

Use

Synthetic Biology

Mutation

WT antiRBS engineered to orthogonal antiRBS, PS e…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED14xW-3

Plasmid

#134806

Purpose

IPTG inducible expression of W-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

W-TAGA-Evo1 qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED1a6

Plasmid

#134787

Purpose

Luciferase reporter for quadruplet codon TAGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-TAGA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-TAGA

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED1a20

Plasmid

#134788

Purpose

Luciferase reporter for quadruplet codon CAGG

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-CAGG

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-CAGG

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pED1a34

Plasmid

#134789

Purpose

Luciferase reporter for quadruplet codon AGGG

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-AGGG

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

357-AGGG

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge