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pGEX-4T1 14-3-3 epsilon K49E

Plasmid

#11945

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

K49E

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pJP_Azul01

Plasmid

#219670

Purpose

Blue light inducible system

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Inserts

similar to T7 RNA polymerase (core)
similar to YF1-fixJ

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

LacIq35 and pJ23105

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJP_Ctrl10

Plasmid

#219672

Purpose

Contains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Inserts

mCherry
lacI

Use

Tags

Expression

Mutation

Promoter

PT3lacO and pR

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJP_Rep00

Plasmid

#219677

Purpose

Light-inducible repressilator (modified from pLPT107). PT3lacO promoter controls the expression of TetR node.

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Insert

PT3lacO

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PT3lacO

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTS1037

Plasmid

#169616

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1108_Tier3(Lenti_inverse)

Plasmid

#169663

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction in inverted direction with polyA (PCMV-5'LTR-Psi-gag-env-A3-pA::A2-pA::A1-pA-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1035

Plasmid

#169614

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1032

Plasmid

#169612

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1031

Plasmid

#169611

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1033

Plasmid

#169613

Purpose

Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1036

Plasmid

#169615

Purpose

Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#13

Plasmid

#176338

Purpose

The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#13

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Use

Tags

GFP(65C)

Expression

Worm

Mutation

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pscAAV-CBh-aSyn-WPRE3-enSV40pA

Plasmid

#194244

Purpose

Expresses human alpha-Synuclein WT

Depositor

Michael J Fox Foundation MJFF

Insert

aSynuclein(WT) (SNCA Human)

Use

AAV

Tags

Expression

Mutation

Promoter

CBh

Available since

Jan. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-IRAlus-Nicn1

Plasmid

#92345

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

Depositor

Ling-Ling Chen, Gordon Carmichael

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Nicn1

Plasmid

#92344

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.

Depositor

Ling-Ling Chen, Gordon Carmichael

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSe-Rp1

Plasmid

#111644

Purpose

For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A

Depositor

Qunxin She

Article

Peng et al Nucleic Acids Res. 2015 Jan;43(1):406-17

Type

Empty backbone

Use

Archaeal expression vector

Tags

Expression

Mutation

Promoter

araS

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pVH233-Tier1-PmPGK1-eYFP

Plasmid

#169522

Purpose

Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC14

Plasmid

#197408

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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