We narrowed to 165,075 results for: addgene

1. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #232929

   Purpose

   Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   OsTir1(F74G)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 74 to Glycine

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

   Plasmid

   #110560

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-lacZ
   

   Expression

   Bacterial

   Promoter

   rhaSR-PrhaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. attB53-Pac-TRE-mCherry-attB53

   Plasmid

   #183611

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   TRE-mCherry
   

   Use

   Rmce

   Promoter

   TRE

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. OmEF1aCas9P2ApuroSB

   Plasmid

   #165484

   Purpose

   Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

   Depositor

   Dietmar Kueltz

   Article

   Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

   Insert

   Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
   

   Use

   CRISPR; Transposon

   Promoter

   Oreochromis mossambicus EF1 alpha promoter (OmEF1…

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

   Plasmid

   #110561

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-aacC1
   

   Expression

   Bacterial

   Promoter

   rhaSR-PrhaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ShuttleMCP

   Plasmid

   #50959

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   uses portion of the mouse cofilin gene as a mouse…

   Available Since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG1-puro-sgATL3-2

    Plasmid

    #109010

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #2 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLG1-puro-sgATL3-5

    Plasmid

    #109011

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL3-sgRNA #5 (ATL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. attB53-Pac-tetO-mCherry-attB53

    Plasmid

    #183612

    Purpose

    RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tetO-mCherry
    

    Use

    Rmce

    Promoter

    tetO

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIC301

    Plasmid

    #69732

    Purpose

    Retroviral expression of Bub1

    Depositor

    Iain Cheeseman

    Insert

    Bub1 (BUB1 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRPK1 (SRPK1A-c100)

    Plasmid

    #98246

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK1A (SRPK1 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid

    #216668

    Purpose

    Expresses membrane-bound mCherry in mammalian cells

    Depositor

    Leonardo Morsut

    Insert

    mCherry
    

    Tags

    PDGFR Transmembrane Domain

    Expression

    Mammalian

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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