We narrowed to 5,654 results for: pCas

1. pCAG-cofilin WT

   Plasmid

   #78295

   Purpose

   Mammalian expression of mouse cofilin fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mRuby3

   Plasmid

   #171154

   Purpose

   Encodes mRuby3 fluorophore

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-WIPI3

   Plasmid

   #42527

   Depositor

   James Hurley

   Article

   Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

   Insert

   WIPI3 (WDR45B Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   gene sequence is codon optimized for expression i…

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Use

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1-SpCas9

   Plasmid

   #117051

   Purpose

   In vitro expression of S. pyogenes Cas9 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   S. pyogenes Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG/HA-hBIG2

   Plasmid

   #226254

   Purpose

   Expresses N-terminally HA-tagged hBIG2 in mammalian cells

   Depositor

   Hye-Won Shin

   Insert

   BIG2 (ARFGEF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-synPCB2.1

   Plasmid

   #139478

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-OSF-PP

   Plasmid

   #154247

   Purpose

   backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Type

   Empty backbone

   Use

   Tags

   FLAG and Strep-Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_EGFP-GFE3

   Plasmid

   #79869

   Purpose

   Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-WIPI4

    Plasmid

    #42528

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    WIPI4 (WDR45 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-HA-Sox2-IP

    Plasmid

    #13459

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-4493NES

    Plasmid

    #138373

    Purpose

    Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-VCre

    Plasmid

    #89575

    Purpose

    Expresses VCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    VCre recombinase
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Limk1

    Plasmid

    #163414

    Purpose

    Expresses LIMK1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Limk1 (Limk1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-PuroDTK.Neo

    Plasmid

    #72307

    Purpose

    PiggyBac Cassette contains puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Insert

    Puromycin-DTK-neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-NRTH

    Plasmid

    #198541

    Purpose

    Expresses human codon-optimized SpCas9-NRTH and blasticidin resistance: EFS promoter-SpCas9-NRTH-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRTH
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCascade_SPS1(PmcCAST)

    Plasmid

    #224926

    Purpose

    Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

    Depositor

    Leifu Chang

    Article

    Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

    Inserts

    Cas5, Cas6, Cas7, Cas8, Cas11
    CRISPR assay for crRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. p276 eSpCas9_2gRNAs_hH11

    Plasmid

    #164850

    Purpose

    gRNA vector for targeting human H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

    Insert

    gRNAs for targeting human H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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