Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types.
Wu WY, Jackson SA, Almendros C, Haagsma AC, Yilmaz S, Gort G, van der Oost J, Brouns SJJ, Staals RHJ
CRISPR J. 2022 Aug;5(4):536-547. doi: 10.1089/crispr.2021.0150. Epub 2022 Jul 12. PubMed Article
Wu WY, Jackson SA, Almendros C, Haagsma AC, Yilmaz S, Gort G, van der Oost J, Brouns SJJ, Staals RHJ
CRISPR J. 2022 Aug;5(4):536-547. doi: 10.1089/crispr.2021.0150. Epub 2022 Jul 12. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186438 | pCas4_1_2_VA | Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A) |
| 186439 | pCas4_1_Δ2_VA | Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A) |
| 186440 | pCasΔ4_1_2_VA | Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A) |
| 186441 | pCas-mut4_1_2_VA | Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A) |
| 186442 | pCas-4/1_2_VB | Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B) |
| 186443 | pCas-4/1Δ2_VB | Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B) |
| 186444 | pCas-mut4/1_2_VB | Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B) |
| 186445 | pCas_4(I-U)/1_2_VB | Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens |
| 186446 | pCas12a | Encodes Cas12a from F. novicida (type V-A) |
| 186447 | pdCas12a(RuvC) | Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A) |
| 186448 | pCas12(PI) | Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A) |
| 186449 | pCas12b | Encodes Cas12b from A. acidoterrestris (type V-B) |
| 186450 | pdCas12b(RuvC) | Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B) |
| 186451 | pCas12b(PI) | Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B) |
| 186452 | pTarget | used as source for acquisition of new spacers |
| 186454 | pTarget_PS_VA | pTarget containing protospacer for FnCas12a targeting |
| 186455 | pTarget_priming_VA | pTarget containing protospacer with a mismatch on position 1, for FnCas12a targeting |
| 186456 | pTarget_PS_VB | pTarget containing protospacer for AaCas12b targeting |