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5'-3xFLAG Smarcc2-exon minimal CMV pCDNA5

Plasmid

#212074

Purpose

LR vector for integration of Smarcc2-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Smarcc2-exon (Smarcc2 Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2d+exon minimal CMV pCDNA5

Plasmid

#212065

Purpose

LR vector for integration of Mef2d+exon(21nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2d+exon(21nt) (Mef2d Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Sap30bp-exon minimal CMV pCDNA5

Plasmid

#212072

Purpose

LR vector for integration of Sap30bp-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp-exon (Sap30bp Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Smarcc2+exon minimal CMV pCDNA5

Plasmid

#212075

Purpose

LR vector for integration of Smarcc2+exon(93nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Smarcc2+exon(93nt) (Smarcc2 Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D443N)

Plasmid

#134293

Purpose

Expresses GK5 (isoform 2)-D443N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 443 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2 (D280N)

Plasmid

#134290

Purpose

Expresses GK5 (isoform 2)-D280N mutant in bacteria

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

changed Aspartic Acid 280 to Asparagine

Promoter

T7

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v1

Plasmid

#134281

Purpose

Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (25-287)

Plasmid

#134263

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 25-287

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5 (1-287)

Plasmid

#134262

Purpose

Lentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 amino acids 1-287

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v2

Plasmid

#134260

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 2)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-C-Flag-GK5_v1

Plasmid

#134257

Purpose

Lentivector encoding Flag-tagged GK5 (isoform 1)

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 1; 516 amino acids

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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PASK gRNA (BRDN0001149035)

Plasmid

#76204

Purpose

3rd generation lentiviral gRNA plasmid targeting human PASK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PASK (PASK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PASK gRNA (BRDN0001148005)

Plasmid

#76205

Purpose

3rd generation lentiviral gRNA plasmid targeting human PASK

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PASK (PASK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b(C631S, C643S and C699T)

Plasmid

#52128

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Publication

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

C631S, C643S and C699T

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 T7 Jip1b(471-707)

Plasmid

#52428

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Publication

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-470

Available since

April 29, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(282-707)

Plasmid

#52126

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Publication

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
ePB-Puro_TT-hGATA6-T2A-EGFP-CAAX

Plasmid

#252248

Purpose

A PiggyBac donor vector for Dox-inducible expression of GATA6 and membrane-localized EGFP.

Depositor

Jun Wu

Publication

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

GATA6 (GATA6 Human)

Use

Piggybac transposon donor vector

Tags

T2A-EGFP-CAAX

Expression

Mammalian

Promoter

tight TRE

Available since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG L3mbtl3+exon minimal CMV pCDNA5

Plasmid

#212059

Purpose

LR vector for integration of L3mbtl3+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

L3mbtl3+exon(75nt) (L3mbtl3 Mouse)

Expression

Mammalian

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212081

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Expression

Mammalian

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop-exon_siResist minimal CMV pCDNA5

Plasmid

#212082

Purpose

LR vector for integration of Chtop-exon_siResist into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Publication

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon_siResist (Chtop Mouse)

Expression

Mammalian

Mutation

GCCTATATGTCCAAGACCA

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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