We narrowed to 6,480 results for: poly

1. Arp2/3_pBIG2abcd

   Plasmid

   #173683

   Purpose

   Insect expression of the 7-subunit Human Arp2/3 complex

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Inserts

   Arp2
   Arp3
   ArpC2
   ArpC3-FLAG
   ArpC4
   ArpC5
   ArpC1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacPAK p110a WT

   Plasmid

   #55722

   Purpose

   His3 GST tagged WT p110 alpha

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha (Pik3ca Mouse)
   

   Use

   Tags

   His3 GST

   Expression

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pFastBac-Dual-M2

   Plasmid

   #135584

   Purpose

   High eukaryotic dual protein expression shuttle vector

   Depositor

   Xiao-Wen Cheng

   Article

   Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

   Type

   Empty backbone

   Use

   Transfer vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin of AcMNPV

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)::NL

   Plasmid

   #141289

   Purpose

   NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

   Depositor

   Andrew Millar

   Article

   Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

   Insert

   NLUC
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Engineered for high stability (t1/2 = 11.5 days a…

   Promoter

   promoter for bacteriophage T7 RNA polymerase

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIB

   Plasmid

   #80610

   Purpose

   pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5HT6-YFP-Inp54p

   Plasmid

   #96812

   Purpose

   cilia-membrane-targeted inositol polyphosphate 5-phosphatase

   Depositor

   Takanari Inoue

   Article

   Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

   Insert

   5HT6-YFP-Inp54p
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPLP2S-mCf

   Plasmid

   #229986

   Purpose

   LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.

   Depositor

   Barry Lutz

   Article

   Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

   Type

   Empty backbone

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTP813

   Plasmid

   #113308

   Purpose

   human Mre11 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

   Insert

   Mre11 (MRE11 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFB1_hMSH6

   Plasmid

   #129424

   Purpose

   Expression human MSH6 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MSH6 (MSH6 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 7MEQ

    Plasmid

    #176412

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    8 x His Tag and Honeybee melittin signal

    Expression

    Mutation

    etmprss2_S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available since

    Oct. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 Synthetic, norovirus)
    

    Use

    Tags

    SpyTag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-ACTU−

    Plasmid

    #198869

    Purpose

    Optogenetic recruitment construct to increase inner membrane surface charge

    Depositor

    Peter Devreotes

    Article

    Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

    Insert

    Rad17 polyanionic tail (-14)
    

    Use

    Synthetic Biology

    Tags

    CRY2PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD24-sfGFPx2

    Plasmid

    #51559

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    GLESTCRHASLAVLADERRFSA and MARARA

    Expression

    Bacterial

    Mutation

    Contains additonal aa in the N and C terminus (in…

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. DCAF1:PBC020-D05:C246337

    Plasmid

    #183974

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV and MHHHHHHSSGRENLYFQG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pFB1_hPMS2

    Plasmid

    #129425

    Purpose

    Expression human PMS2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human PMS2 (PMS2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1_hMLH1

    Plasmid

    #129426

    Purpose

    Expression human MLH1 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MLH1 (MLH1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB1_hMSH2

    Plasmid

    #129423

    Purpose

    Expression human MSH2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH2 (MSH2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0040-LwaCas13a crRNA backbone

    Plasmid

    #103851

    Purpose

    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-LwaCas13aDR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Col2.3deltaTK

    Plasmid

    #114273

    Purpose

    Conditional ablation of mature bone cells via ganciclovir exposure of cells expressing a truncated thymidine kinase.

    Depositor

    David Rowe

    Article

    Visnjic et al J Bone Miner Res. 2001 Dec;16(12):2222-3

    Insert

    Col2.3 promoter + deltaTK + BGH polyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2.3 kb rat type 1 collagen

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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