We narrowed to 6,480 results for: poly

1. pEHlyA5

   Plasmid

   #168298

   Purpose

   Secretion of nanobodies and other polypeptides fused to C-terminal secretion signal of HlyA

   Depositor

   Luis Ángel Fernández

   Article

   Ruano-Gallego et al Microb Cell Fact. 2019 Mar 11;18(1):47.

   Insert

   N‑terminal His tag, VHH, HA and E‑tags, C‑HlyA
   

   Use

   Tags

   E-tag, HA-tag, His-tag, Prescission site, and VHH…

   Expression

   Bacterial

   Mutation

   Promoter

   LacI-Plac

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM3

   Plasmid

   #154168

   Purpose

   Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVasa-Cre

   Plasmid

   #15885

   Depositor

   Diego Castrillon

   Article

   Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

   Insert

   mouse vasa promoter (Ddx4 Mouse)
   

   Use

   Tags

   Cre

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK1043

   Plasmid

   #8835

   Depositor

   David Waugh

   Article

   Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

   Insert

   tobacco etch virus protease, catalytic domain
   

   Use

   Tags

   MBP and polyarginine

   Expression

   Bacterial

   Mutation

   Autolysis-resistant S219V mutant

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ H2B-mCherry

   Plasmid

   #99265

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Article

   Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

   Insert

   mCherry
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTK4_002

   Plasmid

   #123811

   Purpose

   Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   SV40 pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-mCherry-CAAX

   Plasmid

   #166229

   Purpose

   Lentiviral plasmid for expression of mCherry with a C-terminal CAAX polybasic sequence from KRras targeting mCherry to the plasma membrane.

   Depositor

   Carlos Carmona-Fontaine

   Article

   Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

   Insert

   mCherry-CAAX
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTM1_VP35_ZEBOV

   Plasmid

   #68121

   Purpose

   Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   VP35 ZEBOV (VP35 )
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. RNF168-Halo HRD

   Plasmid

   #207080

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTM1_VP30_ZEBOV

    Plasmid

    #69119

    Purpose

    Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

    Insert

    VP30 ZEBOV (VP30 )
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5M-PR-A

    Plasmid

    #89124

    Purpose

    mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-A (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

    Plasmid

    #176630

    Purpose

    Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pSmart-EF1a-Nla-cas11

    Plasmid

    #178882

    Purpose

    Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas11c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2,0_3E5E_eGFP

    Plasmid

    #69359

    Purpose

    Encodes for Ebola Minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    3E5E eGFP minigenome
    

    Use

    T7

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRN3P_T3_ABEmax_IVT

    Plasmid

    #171761

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    ABEmax
    

    Use

    Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS_Transposase

    Plasmid

    #109768

    Purpose

    Codon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Tol2 Transposase
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRN3P_T3_BE4_IVT

    Plasmid

    #171762

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE4
    

    Use

    Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDctp-mSox10-Mp1

    Plasmid

    #13513

    Depositor

    William Pavan

    Article

    Hakami et al Mech Dev. 2006 Feb . 123(2):124-34.

    Insert

    Sox10 (Sox10 Mouse)
    

    Use

    Tags

    Dct promoter and Mp1-polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. HPRT

    Plasmid

    #22884

    Depositor

    James Thomson

    Article

    Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

    Insert

    hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)
    

    Use

    Cre/Lox; Homologous recombination

    Tags

    Expression

    Mutation

    PGK, NeoR, and BGH polyA are between the two geno…

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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