We narrowed to 15,732 results for: LEA

1. pICSL11059

   Plasmid

   #68263

   Purpose

   Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-Raichu-454HRasCT

   Plasmid

   #176029

   Purpose

   A FRET biosensor for HRas activity

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   Raichu-Ras-HRasCT
   

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWF079_LVt-EF1a-TCRGibson_humanTRAC

   Plasmid

   #233448

   Purpose

   Gibson assembly of TCRB-P2A-TCRa

   Depositor

   William Freed-Pastor

   Article

   Ely et al Science. 2025. May 8; 388(6747)

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-BHMT-I/G

   Plasmid

   #104442

   Purpose

   Autophagy cargo assay

   Depositor

   Carol Mercer

   Article

   Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

   Insert

   BHMT (BHMT Human)
   

   Tags

   GFP-myc GFP is from Aequorea victoria and GST

   Expression

   Mammalian

   Mutation

   N43A in GST (in a potential KFERQ-like sequence).…

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b CnA CnB

   Plasmid

   #11787

   Depositor

   Anjana Rao

   Article

   Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

   Insert

   CnA CnB (PPP3R1, PPP3CA Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. YPET-GGA3

   Plasmid

   #18841

   Depositor

   Martin Schwartz

   Article

   Hall et al Anal Biochem. 2008 Mar 15. 374(2):243-9.

   Insert

   YPET-GGA3 (GGA3 Human)
   

   Tags

   His6 and myc

   Expression

   Mammalian

   Mutation

   The fluorescent protein YPET was added to the ami…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Ad-Flag-XBP1s

   Plasmid

   #63678

   Purpose

   Adenoviral vector for XBP1s expression

   Depositor

   Lingfang Zeng, Qingbo Xu

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-CKAR2

    Plasmid

    #123348

    Purpose

    Improved FRET-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    CKAR2
    

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK351

    Plasmid

    #129696

    Purpose

    For insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435

    Depositor

    John Heap

    Article

    Kelly et al Microorganisms. 2019 Aug 16;7(8). pii: m

    Inserts

    PrhaBAD
    yfp
    rhaS
    Terminator ECK120034435
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDarmo.CMVT_v1

    Plasmid

    #133072

    Purpose

    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-Flag-TwinStrep and TwinStrep-Flag-3C

    Expression

    Mammalian

    Promoter

    CMVT (Enhanced CMV)

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-STIM1

    Plasmid

    #228952

    Purpose

    Retroviral expression of mCherry-STIM1

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-GfaABC1D-GRAB_Ado1.0med

    Plasmid

    #153289

    Purpose

    Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Adenosine sensor GRAB_Ado1.0med (medium affinity)
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR_L1-YFP-miniTurbo-L2

    Plasmid

    #127352

    Purpose

    gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and YFP

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-SUMO-hDDI2_pET11a

    Plasmid

    #169120

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKII-GRAB_g5-HT3.0

    Plasmid

    #208723

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in excitatory neurons

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    CaMKII

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA1 Flag ATXN1[2Q]

    Plasmid

    #33235

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    CMV

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-DIO(ChRger2-TS-YFP)

    Plasmid

    #127239

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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