We narrowed to 11,788 results for: nsf

1. PPDK

   Plasmid

   #64037

   Purpose

   Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

   Depositor

   Vern Schramm

   Article

   Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

   Insert

   pyruvate phosphate dikinase
   

   Tags

   none

   Expression

   Bacterial

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-TRE-tdTomato-WPRE

   Plasmid

   #104112

   Purpose

   AAV-mediated expression of tdTomato under the TRE promoter.

   Depositor

   Takeshi Imai

   Article

   Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-GCaMP6s

   Plasmid

   #135418

   Purpose

   Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

   Depositor

   Rylan Larsen

   Insert

   GCaMP6s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-GFP

   Plasmid

   #58867

   Purpose

   AAV expression of GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

   Insert

   GFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CamK2a(0.4)-Opto-cytTrkB(E281A)-HA

   Plasmid

   #180589

   Purpose

   Opto-cytTrkB

   Depositor

   Won Do Heo

   Article

   Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

   Insert

   TrkB
   

   Use

   AAV

   Mutation

   PHR(E281A)

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-UAS-CITRINE

   Plasmid

   #104839

   Purpose

   SPARK reporter gene

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   Citrine
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   UAS

   Available Since

   Jan. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-FUW-eGFP

   Plasmid

   #73083

   Purpose

   Lentiviral transfer vector for tet-inducible expression of EGFP

   Depositor

   Andrew Putnam

   Article

   Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-HA::UPRT

   Plasmid

   #47110

   Purpose

   for subcloning UPRT into expression vectors

   Depositor

   Mike Cleary, Chris Doe

   Article

   Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

   Insert

   uracil phosphoribosyltransferase
   

   Tags

   HA

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEEK2

    Plasmid

    #83492

    Purpose

    Expression plasmid for conjugational transfer into cyanobacteria

    Depositor

    Pia Lindberg

    Article

    Miao et al Metab Eng Commun. 2017 Jul 29;5:45-53. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Strep-tag

    Expression

    Bacterial

    Promoter

    Ptrc core

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST50Trc1-HISNDHFR

    Plasmid

    #63940

    Purpose

    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST50Trc3-HISNDHFR

    Plasmid

    #63976

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST50Trc4-HISNDHFR

    Plasmid

    #63994

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST50Trc2-HISNDHFR

    Plasmid

    #63958

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    6xHis; TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK-N. tabacum atpB/accD

    Plasmid

    #123582

    Purpose

    MoChlo kit

    Depositor

    Scott Lenaghan, C. Neal Stewart

    Article

    Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

    Insert

    N. tabacum atpB/accD integration site
    

    Use

    Chloroplast transformation vector

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX-IRES-GFP

    Plasmid

    #65436

    Purpose

    For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    IRES-GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1a-DIO-oChIEF(E163A/T199C)-P2A-dTomato-WPRE-BGHpA

    Plasmid

    #51094

    Purpose

    Cre dependent expression of oChIEF kinetic variant E163A/T199C with physically uncoupled dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    oChIEF(E163A/T199C)
    

    Use

    AAV

    Tags

    P2A-dTomato

    Expression

    Mammalian

    Mutation

    E163A/T199C

    Promoter

    EF1a

    Available Since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human USP30 (1-517, WT, MG-38-11)

    Plasmid

    #110748

    Purpose

    Transient transfection of human USP30

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid

    #107737

    Purpose

    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

    Depositor

    Rylan Larsen

    Insert

    synaptophysin-GCaMP6s, mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Stuffer

    Plasmid

    #106248

    Purpose

    Destination vector for U6::gRNA expression cassette

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Type

    Empty backbone

    Use

    AAV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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