We narrowed to 10,393 results for: plasmids 101

1. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBstSK+IRES-V5-IPpoI

   Plasmid

   #206321

   Purpose

   A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

   Depositor

   Brian McStay

   Article

   van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

   Insert

   IRES element-V5 epitope tag- IPpoI open reading frame
   

   Use

   Mrna production

   Tags

   V5-IPpoI fusion protein

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA2_GAL4UAS-Luciferase reporter

   Plasmid

   #64158

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. B108-Otx2-IRES-Cre

   Plasmid

   #73993

   Purpose

   B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
   

   Expression

   Mammalian

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLENTI CMV GFP-TREX1 V235fs BLAST

   Plasmid

   #164236

   Purpose

   lentiviral plasmid for GFP-TREX1 V235fs

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   V235 frame shift mutation

   Promoter

   CMV

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZNp-2

   Plasmid

   #245373

   Purpose

   Negative control, firefly luciferase mutated

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Insert

   Zfp423-wt (Zfp423 Mouse)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   NanoLuciferase

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ_pF-3

   Plasmid

   #245374

   Purpose

   Negative control, NanoLuciferase mutated

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Insert

   Zfp423-wt (Zfp423 Mouse)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   NanoLuciferase

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNpF-wt

   Plasmid

   #245364

   Purpose

   Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

   Depositor

   Bruce Hamilton

   Article

   Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

   Insert

   Zfp423-wt (Zfp423 Mouse)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   NanoLuciferase

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Bxb1-GA-CpG-free-mStayGold

   Plasmid

   #241386

   Purpose

   Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mStayGold
   

   Expression

   Mammalian

   Promoter

   CpG-free

   Available Since

   Sept. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221818

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT7R homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor

    Plasmid

    #221819

    Purpose

    Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    GFP11-HA donor with homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTwist Amp_d5-HT1AR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221832

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1AR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT1AR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221833

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2BR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT2BR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist Amp_d5-HT1BR-7xsfGFP11-HA-HDR-donor

    Plasmid

    #221837

    Purpose

    Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT1BR coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    7xGFP11-HA donor with 5-HT1BR homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_CK8e_Cas9_miniPA

    Plasmid

    #226141

    Purpose

    AAV construct for HITI insert with CK8e promoter and SpCas9

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CK8e

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

    Plasmid

    #239294

    Purpose

    Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 promoter
    ITS-tRNAIle intron
    T7 terminator - Var1
    

    Expression

    Yeast

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid

    #229800

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; mRuby2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    hEF1a and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMART-HCKan-yibDp-VHH-GFPenhancer

    Plasmid

    #202468

    Purpose

    Expresses the nanobody GFP enhancer after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP enhancer
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMART-HCKan-yibDp-VHH-GFPminimizer

    Plasmid

    #202469

    Purpose

    Expresses the nanobody GFP minimizer after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody GFP minimizer
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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