161,152 results

1. mCh-alpha-tubulin

   Plasmid

   #49149

   Purpose

   expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

   Depositor

   Gia Voeltz

   Article

   Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

   Insert

   alpha tubulin (TUBA1B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1-Luc2-P2A-tdTomato

   Plasmid

   #72486

   Purpose

   Express Luc2 and tdTomato under EF1 promoter

   Depositor

   Kazuhiro Oka

   Insert

   Luc2-P2A-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCB306

   Plasmid

   #89360

   Purpose

   sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   GFP, puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHREAC

   Plasmid

   #134908

   Purpose

   Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

   Depositor

   George Lomonossoff

   Article

   Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   double 35S

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-CAG -IRES-GFP

   Plasmid

   #69047

   Purpose

   Lentiviral backbone for GFP coexpression

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet_Giantin_C1

   Plasmid

   #85048

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-307_LgBiT

   Plasmid

   #191211

   Purpose

   Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

   Depositor

   Mohan Babu

   Insert

   LgBiT (large bit)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   AB030246.1 (mutated in PMID 26569370)

   Promoter

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-DsRed

   Plasmid

   #11151

   Purpose

   Mammalian expression vector for expression of DsRed

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   DsRed2 from Discosoma sp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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