We narrowed to 13,360 results for: Mpl;

1. pFSW Syt1-C2B V304A,I367A IRES GFP

   Plasmid

   #133821

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   V304A, I367A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFSW Syt1-C2A K189-192A IRES GFP

   Plasmid

   #133823

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   K189-192A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSW Syt1-C2A D230,232N IRES GFP

   Plasmid

   #133824

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D230,232N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSW Syt1-C2A M173,F234A IRES GFP

   Plasmid

   #133825

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   M173, F234A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. eeBxb1_IVT

   Plasmid

   #222342

   Purpose

   Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   Template for in vitro transcription

   Promoter

   T7 (inactivated)

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEGFP-Rac1

   Plasmid

   #83950

   Purpose

   Rac1 FLIM donor for dynamic imaging of GTPase activity

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-N-Ezrin

    Plasmid

    #202684

    Purpose

    fluorescent protein tagged ezrin

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus EZR(1-586):EGFP (Ezr Mouse)
    

    Expression

    Mammalian

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti dCAS-VP64_Blast

    Plasmid

    #61425

    Purpose

    3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Lentiviral Prep

    Insert

    dCAS9(D10A, N863A)-VP64_2A_Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS117 His14-Avi-45xGS-anti GFP nanobody

    Plasmid

    #199370

    Purpose

    Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-GFP nanobody
    

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5' Del 1

    Plasmid

    #16049

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1372 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. 5' Del 2

    Plasmid

    #16050

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Id1 promoter, 5' deletion (Id1 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    -1147 to +88 of promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4-Chk1-Flag

    Plasmid

    #22894

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Chk1 (CHEK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-hMALAT1

    Plasmid

    #118580

    Purpose

    to overexpress human MALAT1 in mammalian cells

    Depositor

    Jianjun Zhao

    Article

    Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

    Insert

    MALAT1 (MALAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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