We narrowed to 10,783 results for: T7
-
Plasmid#67134PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only
-
pfMDH_K381A
Plasmid#67732PurposeExpresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged lysine 381 to alaninePromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N300D
Plasmid#67728PurposeExpresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 300 to aspartatePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_R373A
Plasmid#67731PurposeExpresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged arginine 373 to alaninePromoterT7Available SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_N300S
Plasmid#67729PurposeExpresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
Tags6x-His tagExpressionBacterialMutationChanged asparagine 300 to serinePromoterT7Available SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119(T214V)
Plasmid#66137PurposeExpresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
TagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationT214VPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 T180C
Plasmid#66181PurposeExpresses the T180C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationT180CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA3 R112C
Plasmid#66180PurposeExpresses the R112C mutant of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
TagsHis6 tagExpressionBacterialMutationR112CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 E105C
Plasmid#66176PurposeExpresses the E105C mutant of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
TagsHis6 tagExpressionBacterialMutationE105CPromoterT7 promoterAvailable SinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN491
Plasmid#62594PurposeTALEN array receiving vector with half-site NN RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN443
Plasmid#62593PurposeTALEN array receiving vector with half-site NG RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN442
Plasmid#62591PurposeTALEN array receiving vector with half-site HD RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN441
Plasmid#62590PurposeTALEN array receiving vector with half-site NI RVDDepositorTypeEmpty backboneUseTALEN; T7Tags3× FLAG and FOKI WTExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAD06 K. lactis telomerase RNA hairpin
Plasmid#61895PurposePlasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)DepositorInsertTelomerase RNA hairpin (nt 229-273)
PromoterT7Available SinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Guinea Pig 1-88)
Plasmid#62059PurposeExpression of Guinea Pig p53 (residues 1-88) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-88PromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap
Plasmid#49160PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gapDepositorInsertBacteriophage T4 gene 60
Mutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 to gap + Cys
Plasmid#49159PurposeTemplate for generating gene 60 mRNA, sequence 5' of coding gap + CysDepositorInsertBacteriophage T4 gene 600
MutationCoding gap and downstream sequence have been dele…PromoterT7Available SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpDJ-1 pET15b
Plasmid#60689PurposeRecombinant protein expression of DJ-1DepositorAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only