We narrowed to 22,283 results for: fus

1. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

   Plasmid

   #130990

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

   Plasmid

   #130997

   Purpose

   Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   EYFP-Kv2.1

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. NUAK1 gRNA (BRDN0001162598)

   Plasmid

   #75577

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUAK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NUAK1 (NUAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. NUAK1 gRNA (BRDN0001147733)

   Plasmid

   #75576

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUAK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NUAK1 (NUAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NUAK1 gRNA (BRDN0001162525)

   Plasmid

   #75578

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NUAK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NUAK1 (NUAK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hA3A-BE3-Y130F

   Plasmid

   #113428

   Purpose

   Expresses hA3A-BE3-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PHKG1 gRNA (BRDN0001144981)

   Plasmid

   #76356

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PHKG1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PHKG1 (PHKG1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-HA active Nedd8-P2A-Hygro

   Plasmid

   #124307

   Purpose

   Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Five C-terminal amino acids missing compared to w…

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKN3 gRNA (BRDN0001146586)

    Plasmid

    #77947

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS-CG-WT-Cer-VWF

    Plasmid

    #124827

    Purpose

    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. STK17A gRNA (BRDN0001149480)

    Plasmid

    #77916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17A

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17A (STK17A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. STK17B gRNA (BRDN0001146764)

    Plasmid

    #76798

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK17B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK17B (STK17B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PFKFB4 gRNA (BRDN0001149495)

    Plasmid

    #77123

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PFKFB4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PFKFB4 (PFKFB4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-syn-SnFR-gamma2-minWPRE

    Plasmid

    #165497

    Purpose

    AAV for glutamate reporter fused to TARP gamma-2 for postsynaptic localisation

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Synthetic, Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PKN3 gRNA (BRDN0001146792)

    Plasmid

    #77948

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PKN3 gRNA (BRDN0001146747)

    Plasmid

    #77949

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PKN3 gRNA (BRDN0001487150)

    Plasmid

    #77950

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN3 (PKN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBMN Parkin

    Plasmid

    #89299

    Purpose

    For expression of untagged Parkin

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-C1-FKBP-CYB5tail

    Plasmid

    #202686

    Purpose

    rapamycin-induced recruitment of proteins to ER

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-hA3C-BE3

    Plasmid

    #113412

    Purpose

    Expresses hA3C-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3C-BE3 (APOBEC3C Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLC-CUL4A WT-P2A-Puro

    Plasmid

    #124304

    Purpose

    Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. kiCAP-AAV-M.Mus.2

    Plasmid

    #196682

    Purpose

    Rep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    REQQKLW insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130993

    Purpose

    AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. non-targeting control gRNA (BRDN0001146004)

    Plasmid

    #80174

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge