We narrowed to 40,052 results for: lat

1. pPL5618_pUG_MtxR

   Plasmid

   #60929

   Purpose

   PCR template vector for generating methotrexate resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   Dihydrofolate reductase (DHFR Human)
   

   Use

   Loxp flanked deletion cassette template

   Mutation

   L22F F31S

   Promoter

   TEF1

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STAGR_SAMScaffold_hU6

   Plasmid

   #102843

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   As pcr template

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE-UBC-TagRFP-T-ps

   Plasmid

   #178656

   Purpose

   Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

   Insert

   TagRFP-T with palmitoylation signal at N-terminus
   

   Use

   Lentiviral

   Tags

   Palmitoylation signal

   Promoter

   Ubc

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-HA-C7orf59

   Plasmid

   #42336

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   C7orf59 (LAMTOR4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

   Plasmid

   #115487

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LRP6N-IgG-pCS2

   Plasmid

   #27279

   Depositor

   Xi He

   Article

   Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

   Insert

   low density lipoprotein receptor-related protein 6 (LRP6 Human)
   

   Use

   Xenopus injection

   Tags

   IgG Fc

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pEG302 22aa SunTag VP64 g4+g17

   Plasmid

   #120252

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PERK 4: PERKKD-pGEX4T-1

   Plasmid

   #21817

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (537-1114) (Eif2ak3 Mouse)
   

   Tags

   GST and Myc (9E10)

   Expression

   Bacterial

   Mutation

   PERK Kinase Domain. Amino acids 537-1114.

   Available Since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMIEG3-BCL6

    Plasmid

    #40339

    Depositor

    Alexander Dent

    Article

    Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Retroviral

    Tags

    IRES eGFP

    Expression

    Mammalian

    Mutation

    optimized translation initiation codon

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA HisB TO rFez1

    Plasmid

    #17385

    Depositor

    Stephen Goff

    Article

    Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

    Insert

    FEZ1 (Fez1 Rat)
    

    Tags

    his and myc

    Expression

    Mammalian

    Available Since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV_tKiMBI-T2A-caMEK

    Plasmid

    #199578

    Purpose

    Expresses tKiMBI and caMEK in an AAV vector

    Depositor

    Michael Lin

    Article

    Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

    Inserts

    ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
    constitutively active MEK
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFAB808

    Plasmid

    #47853

    Purpose

    BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rrnB T1 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLTetO8

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

    Plasmid

    #135412

    Purpose

    simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    SomArchon-EGFP-P2A-stGtACR2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p5E-pth2

    Plasmid

    #191428

    Purpose

    Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

    Depositor

    Timothy Erickson

    Article

    Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

    Insert

    pth2 regulatory region (pth2 Zebrafish)
    

    Use

    Tol2 gateway 5' entry vector

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-gRNA-puro

    Plasmid

    #180426

    Purpose

    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    gRNAscr
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge