We narrowed to 9,184 results for: Pol;

1. A3Bctd-D314E-Cas9n-UGI-NLS

   Plasmid

   #198887

   Purpose

   Express A3Bctd-D314E BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D314E

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eA3Ai-Cas9n-UGI-NLS

   Plasmid

   #207165

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Tags

   Cas9-UGI--NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. eA3Ai-max

   Plasmid

   #207166

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Tags

   Cas9-UGI-UGI-NLS and NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMVQA

   Plasmid

   #197894

   Purpose

   pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

   Depositor

   Thomas Klimkait

   Article

   Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

   Insert

   HIV-1 3' end (HXB2: 9045 till poly-A(10)
   

   Expression

   Bacterial

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-DIO-basic

   Plasmid

   #196882

   Purpose

   Used for preparation of double-floxed inverse ORF vectors

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Type

   Empty backbone

   Use

   Cre/Lox; Dre/rox

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBactin-mOrange2-CI

   Plasmid

   #196852

   Purpose

   Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

   Plasmid

   #197969

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   L1332V, delta(1616-1666)

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

   Plasmid

   #197970

   Purpose

   Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   C328S, C331S, L1332V, delta(1616-1666)

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. H1.M10.luc

    Plasmid

    #193706

    Purpose

    Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV.CBA.YFP.miR-E_shSrpk3-1

    Plasmid

    #180400

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-1 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV.CBA.YFP.miR-E_shSrpk3-3

    Plasmid

    #180402

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-3 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV.CBA.YFP.miR-E_shStk16-2

    Plasmid

    #180392

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-2 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV.CBA.YFP.miR-E_shStk16-3

    Plasmid

    #180393

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-3 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL5.0-EGFP-HRasC20

    Plasmid

    #187252

    Purpose

    Lenticiral expression of EGFP-HRasC20

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi:

    Insert

    HRasC20 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    5' LTR

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pST_YlHD (pBS0275)

    Plasmid

    #185153

    Purpose

    For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    YlHD
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Expression

    Bacterial

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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